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PMID: 8849266 Published · ppublish English Comparative Study Journal Article

Clinical strain of Staphylococcus aureus inactivates and causes efflux of macrolides.

Antimicrobial agents and chemotherapy ·Vol. 40 ·No. 4 ·1996-04-00 ·Pages 992-8

Wondrack L, Massa M, Yang BV, Sutcliffe J

Abstract

Searching through a collection of 124 Staphylococcus aureus clinical strains, we found one isolate, strain 01A1032, that inactivates 14- and 16-membered macrolides. The products of inactivation were purified from supernatant fluids of cultures exposed to erythromycin for 3 h and were found to be identical to products of inactivation from Escherichia coli strains that encode either an EreA or EreB esterase. Further, strain 01A1032 was shown to be resistant to azithromycin, a 15-membered macrolide, by an alternate mechanism, efflux. Thus, strain 01A1032 harbors determinants encoding an esterase activity that hydrolyzes 14- and 16-membered macrolides and a macrolide efflux system.

MeSH Terms
Anti-Bacterial Agents/metabolism Azithromycin/metabolism Base Sequence Drug Resistance, Microbial Erythromycin/metabolism Escherichia coli/metabolism Molecular Sequence Data Staphylococcus aureus/metabolism
Chemicals
Anti-Bacterial Agents Erythromycin Azithromycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wondrack L
Department of Infectious Diseases, Central Research Division, Pfizer, Inc., Groton, Connecticut 06340, USA.
Massa M
Yang B V
Sutcliffe J
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.40.4.992
Published
1996-04-00
Pages
992-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC163245
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.40.4.992 PMC Full Text View journal details
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