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PMID: 1406590 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization and nucleotide sequence of the oriT-traM-finP region of the IncFVII plasmid pSU233.

Molecular & general genetics : MGG ·Vol. 234 ·No. 3 ·1992-09-00 ·Pages 442-8

Salazar L, Lopéz J, Andrés I, Ortiz JM, Rodríguez JC

Abstract

By hybridizing the IncFVII haemolytic plasmid pSU233 with a probe containing the origin of transfer of the IncFII plasmid R1, we isolated a 1.9 kb BglII fragment containing at least the origin of transfer (oriT), and the genes traM and finP. Functional complementation analysis of deletion derivatives was used to map the origin of transfer. We also determined the nucleotide sequence of traM and finP. Comparison with similar regions of several plasmids, also belonging to the Rep-FIIA family, revelaed that pSU233 resembles the F plasmid by very close. The homology is not evenly distributed along this region, but clustered into homologous regions (TraZb-oriT, TraMb-oriT and traM separated by non-homologous regions (TraYb-oriT, finP). This organization resembles that reported for the replication region and also suggests evolution by exchange of modules. In addition, the nucleotide sequence of finP is different from those previously described for other IncF plasmids and constitutes a new allele, which we have denominated allele VI.

Related Genes
finP oriT traM traY
MeSH Terms
Amino Acid Sequence Base Sequence DNA Replication DNA, Bacterial/genetics F Factor Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Restriction Mapping Sequence Alignment Transduction, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Salazar L
Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
Lopéz J
Andrés I
Ortiz J M
Rodríguez J C
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00538704
Published
1992-09-00
Pages
442-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X55896
External Links
PubMed source DOI: 10.1007/BF00538704
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