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PMID: 1538696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and sequence analysis of cdc27+ required for the G2-M transition in the fission yeast Schizosaccharomyces pombe.

Molecular & general genetics : MGG ·Vol. 231 ·No. 3 ·1992-02-00 ·Pages 401-10

Hughes DA, MacNeill SA, Fantes PA

Abstract

The cell division cycle gene cdc27+ of the fission yeast Schizosaccharomyces pombe is required for the transition from G2 into mitosis. Genetic and physiological experiments suggest a close relationship between cdc27+ and the cdc2+ gene, a key regulator of mitosis in yeast and also in higher eukaryotic cells. We isolated the cdc27+ gene by complementation of a temperature-sensitive cdc27 mutant. The DNA sequence of this gene predicts a 1116 nucleotide open reading frame split by five short introns, ranging in size from 49 to 74 nucleotides. Analysis of cDNA clones confirmed the structure of the gene. The deduced cdc27+ gene product consists of 372 amino acids with a predicted Mr of 43 kDa. No homology of the predicted protein with known proteins could be found, thus the cdc27+ gene encodes a novel function required for the G2-M transition. Northern analysis revealed two mRNAs of 1.4 and 2.2 kb transcribed from this gene, the smaller transcript being approximately tenfold more abundant than the larger. The level of cdc27+ mRNAs remained constant through the cell cycle indicating that the time of action of the cdc27+ gene, which is known to be regulated by elements of the mitotic control, is not determined by periodic accumulation of its transcripts.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cell Cycle Proteins Chromosome Deletion Cloning, Molecular DNA Polymerase III DNA, Fungal/genetics,isolation & purification Fungal Proteins/genetics,metabolism G2 Phase Genetic Complementation Test Introns Mitosis Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction RNA Splicing RNA, Fungal/genetics,isolation & purification RNA, Messenger/genetics,isolation & purification Schizosaccharomyces/cytology,genetics,growth & development Schizosaccharomyces pombe Proteins Transcription, Genetic
Chemicals
Cell Cycle Proteins DNA, Fungal Fungal Proteins Oligodeoxyribonucleotides RNA, Fungal RNA, Messenger Schizosaccharomyces pombe Proteins cdc27 protein, S pombe DNA Polymerase III
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hughes D A
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
MacNeill S A
Fantes P A
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00292709
Published
1992-02-00
Pages
401-10
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
M74062, M83307
External Links
PubMed source DOI: 10.1007/BF00292709
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