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PMID: 15890959 Published · ppublish English Journal Article

Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor.

Journal of virology ·Vol. 79 ·No. 11 ·2005-06-00 ·Pages 7207-16

Belov GA, Fogg MH, Ehrenfeld E

Abstract

Poliovirus infection results in the disintegration of intracellular membrane structures and formation of specific vesicles that serve as sites for replication of viral RNA. The mechanism of membrane rearrangement has not been clearly defined. Replication of poliovirus is sensitive to brefeldin A (BFA), a fungal metabolite known to prevent normal function of the ADP-ribosylation factor (ARF) family of small GTPases. During normal membrane trafficking in uninfected cells, ARFs are involved in vesicle formation from different intracellular sites through interaction with numerous regulatory and coat proteins as well as in regulation of phospholipase D activity and cytoskeleton modifications. We demonstrate here that ARFs 3 and 5, but not ARF6, are translocated to membranes in HeLa cell extracts that are engaged in translation of poliovirus RNA. The accumulation of ARFs on membranes correlates with active replication of poliovirus RNA in vitro, whereas ARF translocation to membranes does not occur in the presence of BFA. ARF translocation can be induced independently by synthesis of poliovirus 3A or 3CD proteins, and we describe mutations that abolished this activity. In infected HeLa cells, an ARF1-enhanced green fluorescent protein fusion redistributes from Golgi stacks to the perinuclear region, where poliovirus RNA replication occurs. Taken together, the data suggest an involvement of ARF in poliovirus RNA replication.

MeSH Terms
ADP-Ribosylation Factor 1/genetics,metabolism ADP-Ribosylation Factors/metabolism Biological Transport, Active/drug effects Brefeldin A/pharmacology Green Fluorescent Proteins/genetics,metabolism HeLa Cells Humans In Vitro Techniques Intracellular Membranes/metabolism,virology Mutation Poliovirus/genetics,pathogenicity,physiology RNA, Viral/metabolism Recombinant Fusion Proteins/genetics,metabolism Viral Proteins/genetics,physiology Virus Replication
Chemicals
RNA, Viral Recombinant Fusion Proteins Viral Proteins enhanced green fluorescent protein Green Fluorescent Proteins Brefeldin A ADP-Ribosylation Factor 1 ADP-Ribosylation Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Belov George A
Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6120, Bethesda, MD 20892-8011, USA.
Fogg Mark H
Ehrenfeld Ellie
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.79.11.7207-7216.2005
Published
2005-06-00
Pages
7207-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC1112117
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.79.11.7207-7216.2005 PMC Full Text View journal details
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