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PMID: 16672383 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast.

Molecular biology of the cell ·Vol. 17 ·No. 7 ·2006-07-00 ·Pages 3267-80

Smits GJ, Schenkman LR, Brul S, Pringle JR, Klis FM

Abstract

The yeast cell wall is an essential organelle that protects the cell from mechanical damage and antimicrobial peptides, participates in cell recognition and adhesion, and is important for the generation and maintenance of normal cell shape. We studied the localization of three covalently bound cell wall proteins in Saccharomyces cerevisiae. Tip1p was found only in mother cells, whereas Cwp2p was incorporated in small-to-medium-sized buds. When the promoter regions of TIP1 and CWP2 (responsible for transcription in early G1 and S/G2 phases, respectively) were exchanged, the localization patterns of Tip1p and Cwp2p were reversed, indicating that the localization of cell wall proteins can be completely determined by the timing of transcription during the cell cycle. The third protein, Cwp1p, was incorporated into the birth scar, where it remained for several generations. However, we could not detect any role of Cwp1p in strengthening the birth scar wall or any functional interaction with the proteins that mark the birth scar pole as a potential future budding site. Promoter-exchange experiments showed that expression in S/G2 phase is necessary but not sufficient for the normal localization of Cwp1p. Studies of mutants in which septum formation is perturbed indicate that the normal asymmetric localization of Cwp1p also depends on the normal timing of septum formation, composition of the septum, or both.

MeSH Terms
Carrier Proteins/analysis,metabolism Cell Cycle Cell Wall/metabolism Cytokinesis Glycoproteins/analysis,metabolism Membrane Glycoproteins/analysis,genetics,metabolism Membrane Proteins/analysis,genetics,metabolism Promoter Regions, Genetic Saccharomyces cerevisiae/chemistry,cytology,metabolism Saccharomyces cerevisiae Proteins/analysis,genetics,metabolism Transcription, Genetic
Chemicals
CWP1 protein, S cerevisiae CWP2 protein, S cerevisiae Carrier Proteins Glycoproteins Membrane Glycoproteins Membrane Proteins Saccharomyces cerevisiae Proteins TIP1 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Smits Gertien J
Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, 1018 WV Amsterdam, The Netherlands. [email protected]
Schenkman Laura R
Brul Stanley
Pringle John R
Klis Frans M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e05-08-0738
Email
[email protected]
Published
2006-07-00
Epub
2006-00-03
Pages
3267-80
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1483055
Subset
IM
Grants
NIGMS NIH HHS · R01 GM031006 · United States
NIGMS NIH HHS · R37 GM031006 · United States
NIGMS NIH HHS · GM-31006 · United States
External Links
PubMed source DOI: 10.1091/mbc.e05-08-0738 PMC Full Text View journal details
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