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PMID: 169231 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Kinetics of the onset of catabolite repression in Escherichia coli as determined by lac messenger ribonucleic acid initiations and intracellular cyclic adenosine 3',5'-monophosphate levels.

Journal of bacteriology ·Vol. 123 ·No. 3 ·1975-09-00 ·Pages 946-53

Haggerty DM, Schleif RF

Abstract

The rates of synthesis of beta-galactosidase (EC 3.2.1.23) and the intracellular levels of cyclic 3',5'-adenosine monophosphate (cAMP) soon after the addition of glucose or glycerol to exponentially growing cultures of Escherichia coli have been determined. Within 10 s of its addition, glucose, but not glycerol, lowered the apparent initiation frequency of lac messenger ribonucleic acid. The glucose-generated reduction in initiations is identified as catabolite repression by its reversibility with cAMP. The intracellular cAMP levels respond virtually identically to glucose and glycerol additions. Thus, no correlation was observed between the rate of messenger ribonucleic acid initiation and the level of cAMP.

MeSH Terms
Cyclic AMP/biosynthesis,pharmacology Enzyme Repression Escherichia coli/metabolism Galactosidases/biosynthesis Glucose/pharmacology Glycerol/pharmacology Kinetics Lactose Operon RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis Rifampin/pharmacology
Chemicals
RNA, Bacterial RNA, Messenger Cyclic AMP Galactosidases Glucose Lactose Glycerol Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haggerty D M
Schleif R F
References (31)
31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.123.3.946-953.1975
Published
1975-09-00
Pages
946-53
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235818
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.123.3.946-953.1975 PMC Full Text View journal details
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