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PMID: 16950924 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

TOS9 regulates white-opaque switching in Candida albicans.

Eukaryotic cell ·Vol. 5 ·No. 10 ·2006-10-00 ·Pages 1674-87

Srikantha T, Borneman AR, Daniels KJ, Pujol C, Wu W, Seringhaus MR, Gerstein M, Yi S, Snyder M, Soll DR

Abstract

In Candida albicans, the a1-alpha2 complex represses white-opaque switching, as well as mating. Based upon the assumption that the a1-alpha2 corepressor complex binds to the gene that regulates white-opaque switching, a chromatinimmunoprecipitation-microarray analysis strategy was used to identify 52 genes that bound to the complex. One of these genes, TOS9, exhibited an expression pattern consistent with a "master switch gene." TOS9 was only expressed in opaque cells, and its gene product, Tos9p, localized to the nucleus. Deletion of the gene blocked cells in the white phase, misexpression in the white phase caused stable mass conversion of cells to the opaque state, and misexpression blocked temperature-induced mass conversion from the opaque state to the white state. A model was developed for the regulation of spontaneous switching between the opaque state and the white state that includes stochastic changes of Tos9p levels above and below a threshold that induce changes in the chromatin state of an as-yet-unidentified switching locus. TOS9 has also been referred to as EAP2 and WOR1.

MeSH Terms
Candida albicans/cytology,metabolism Cell Nucleus/metabolism Chromatin Immunoprecipitation Fungal Proteins/genetics,metabolism Gene Deletion Gene Expression Regulation, Fungal Genes, Mating Type, Fungal/genetics Genes, Switch/genetics Genome, Fungal/genetics Genotype Models, Genetic Protein Binding Protein Transport Proto-Oncogene Proteins c-myc/metabolism RNA, Messenger/genetics,metabolism Recombinant Fusion Proteins/metabolism Temperature Transcription, Genetic
Chemicals
Fungal Proteins Proto-Oncogene Proteins c-myc RNA, Messenger Recombinant Fusion Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Srikantha Thyagarajan
Department of Biological Sciences, 302 BBE, The University of Iowa, Iowa City, IA 52242, USA.
Borneman Anthony R
Daniels Karla J
Pujol Claude
Wu Wei
Seringhaus Michael R
Gerstein Mark
Yi Song
Snyder Michael
Soll David R
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.00252-06
Published
2006-10-00
Epub
2006-00-01
Pages
1674-87
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC1595353
Subset
IM
Grants
NCI NIH HHS · R01 CA077808 · United States
NIAID NIH HHS · AI2392 · United States
NCI NIH HHS · R01-CA077808-9-13 · United States
External Links
PubMed source DOI: 10.1128/EC.00252-06 PMC Full Text View journal details
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