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PMID: 17601340 Published · epublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Analysis of septins across kingdoms reveals orthology and new motifs.

BMC evolutionary biology ·Vol. 7 ·2007-07-01 ·Pages 103

Pan F, Malmberg RL, Momany M

Abstract

Septins are cytoskeletal GTPase proteins first discovered in the fungus Saccharomyces cerevisiae where they organize the septum and link nuclear division with cell division. More recently septins have been found in animals where they are important in processes ranging from actin and microtubule organization to embryonic patterning and where defects in septins have been implicated in human disease. Previous studies suggested that many animal septins fell into independent evolutionary groups, confounding cross-kingdom comparison. In the current work, we identified 162 septins from fungi, microsporidia and animals and analyzed their phylogenetic relationships. There was support for five groups of septins with orthology between kingdoms. Group 1 (which includes S. cerevisiae Cdc10p and human Sept9) and Group 2 (which includes S. cerevisiae Cdc3p and human Sept7) contain sequences from fungi and animals. Group 3 (which includes S. cerevisiae Cdc11p) and Group 4 (which includes S. cerevisiae Cdc12p) contain sequences from fungi and microsporidia. Group 5 (which includes Aspergillus nidulans AspE) contains sequences from filamentous fungi. We suggest a modified nomenclature based on these phylogenetic relationships. Comparative sequence alignments revealed septin derivatives of already known G1, G3 and G4 GTPase motifs, four new motifs from two to twelve amino acids long and six conserved single amino acid positions. One of these new motifs is septin-specific and several are group specific. Our studies provide an evolutionary history for this important family of proteins and a framework and consistent nomenclature for comparison of septin orthologs across kingdoms.

MeSH Terms
Amino Acid Motifs/genetics Animals Caenorhabditis elegans Proteins/chemistry,genetics Cell Cycle Proteins/chemistry,classification,genetics Consensus Sequence Conserved Sequence Cytoskeletal Proteins/chemistry,classification,genetics Drosophila Proteins/chemistry,genetics Evolution, Molecular Fungal Proteins/chemistry,classification,genetics Fungi/enzymology,genetics GTP Phosphohydrolases/chemistry,classification,genetics Gene Duplication Humans Insect Proteins/chemistry,genetics Mice Microsporidia/enzymology,genetics Multigene Family Phylogeny Profilins/chemistry,genetics Protein Isoforms/chemistry,genetics Protein Structure, Tertiary Protozoan Proteins/chemistry,genetics Rats Saccharomyces cerevisiae Proteins/chemistry,genetics Sequence Alignment Sequence Homology, Amino Acid Species Specificity Terminology as Topic Zebrafish Proteins/chemistry,genetics
Chemicals
CDC3 protein, S cerevisiae Caenorhabditis elegans Proteins Cell Cycle Proteins Cytoskeletal Proteins Drosophila Proteins Fungal Proteins Insect Proteins Profilins Protein Isoforms Protozoan Proteins Saccharomyces cerevisiae Proteins Zebrafish Proteins GTP Phosphohydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pan Fangfang
Plant Biology Department, University of Georgia, Athens, GA 30602, USA. [email protected] <[email protected]>
Malmberg Russell L
Momany Michelle
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Article Info
Journal
BMC evolutionary biology
Abbr.
BMC Evol Biol
ISSN
1471-2148
DOI
10.1186/1471-2148-7-103
Email
[email protected]
Published
2007-07-01
Epub
2007-00-01
Pages
103
Language
English
Region
England
NLM ID
100966975
PMCID
PMC1931588
Subset
IM
Grants
NIGMS NIH HHS · R01 GM072080 · United States
NIGMS NIH HHS · 5R01GM072080-02 · United States
External Links
PubMed source DOI: 10.1186/1471-2148-7-103 PMC Full Text View journal details
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