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PMID: 1956774 Published · ppublish English Journal Article

Interplay of an original combination of factors: C/EBP, NFY, HNF3, and HNF1 in the rat aldolase B gene promoter.

Nucleic acids research ·Vol. 19 ·No. 22 ·1991-11-25 ·Pages 6145-53

Raymondjean M, Pichard AL, Gregori C, Ginot F, Kahn A

Abstract

The rat aldolase B 5' flanking region (nucleotides - 194 to +41) contains sufficient information for liver-specific expression. A detailed investigation of factors binding to the rat aldolase B 5' flanking region has allowed us to identify three distinct factors that filled different sites of this region (A, B, C). The liver-enriched C/EBP or related factors bind to box C, as demonstrated by the specific interaction with bacterially expressed C/EBP protein. Box B bearing the CCAAT sequence binds the ubiquitous factor NFY. Surprisingly, Box A is able to bind two liver enriched factors, namely HNF1 and HNF3. However, in the context of the intact promoter, as shown by footprinting competition experiments, HNF3 binds solely to this sequence. HNF3, but not HNF1 is a transcriptional activator as demonstrated in the in vitro transcription assay.

MeSH Terms
Animals Base Sequence CCAAT-Enhancer-Binding Proteins Cell-Free System DNA Fingerprinting DNA-Binding Proteins/metabolism Fructose-Bisphosphate Aldolase/genetics Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Molecular Sequence Data Nuclear Proteins/metabolism Oligonucleotides/metabolism Promoter Regions, Genetic Rats Transcription Factors/metabolism Transcription, Genetic
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Hepatocyte Nuclear Factor 1-alpha Hnf1a protein, rat Nuclear Proteins Oligonucleotides Transcription Factors Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-beta Fructose-Bisphosphate Aldolase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Raymondjean M
ICGM, INSERM U. 129, Paris, France.
Pichard A L
Gregori C
Ginot F
Kahn A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.22.6145
Published
1991-11-25
Pages
6145-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329108
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.22.6145 PMC Full Text View journal details
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