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PMID: 1987112 Published · ppublish English Comparative Study Journal Article

Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K-12.

Journal of bacteriology ·Vol. 173 ·No. 1 ·1991-01-00 ·Pages 108-15

Heatwole VM, Somerville RL

Abstract

The mtr gene of Escherichia coli K-12 encodes an L-tryptophan-specific permease. This gene was originally identified through the isolation of mutations in the 69-min region of the chromosome, closely linked to argG. Cells with lesions in mtr display a phenotype of 5-methyltryptophan resistance. The mtr gene was cloned by using the mini-Mu system. The amino acid sequence of Mtr (414 codons), deduced by DNA sequence analysis, was found to be 33% identical to that of another single-component transport protein, the tyrosine-specific permease, TyrP. The hydropathy plots of the two permeases were similar. Possible operator sites for the tyrosine and tryptophan repressors are situated within the region of DNA that is likely to be the mtr promoter.

Related Genes
mdr
MeSH Terms
Amino Acid Sequence Amino Acid Transport Systems Base Sequence Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Drug Resistance, Microbial/genetics Escherichia coli/drug effects,enzymology,genetics Escherichia coli Proteins Genes, Bacterial Membrane Transport Proteins/genetics Molecular Sequence Data Mutagenesis, Site-Directed Phenotype Protein Conformation Restriction Mapping Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Tryptophan/analogs & derivatives,pharmacology
Chemicals
Amino Acid Transport Systems Escherichia coli Proteins Membrane Transport Proteins Saccharomyces cerevisiae Proteins TAT2 protein, S cerevisiae 5-methyltryptophan TnaB protein, E coli Tryptophan
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heatwole V M
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Somerville R L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.1.108-115.1991
Published
1991-01-00
Pages
108-15
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207163
Subset
IM
Databases
GENBANK
M35417, M59862, S68058, S70324, S70325, S70331, S70358, S70359, S70363, S70365, S70370
External Links
PubMed source DOI: 10.1128/jb.173.1.108-115.1991 PMC Full Text View journal details
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