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PMID: 2011599 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genomically imposed and somatically modified human thymocyte V beta gene repertoires.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 7 ·1991-04-01 ·Pages 2908-12

Baccala R, Kono DH, Walker S, Balderas RS, Theofilopoulos AN

Abstract

The effect of thymic selection on the expressed human T-cell antigen receptor beta-chain variable region (V beta) gene repertoire was examined by using a multiprobe RNase protection assay. The relative abundance of transcripts for 22 V beta genes (encompassing 17 of the 20 human V beta gene subfamilies) within a thymus, and among 17 thymuses, was variable. On the basis of the presence of corresponding mRNAs, no genomic deletions were detected, but several coding region polymorphisms were identified. Analysis of mature T-cell subsets revealed the absence of complete "superantigen"-mediated V beta deletions, suggesting that this phenomenon, in contrast to mouse, is uncommon or absent in humans. However, several V beta genes were over- or underexpressed in one or both mature single-positive (CD4+8- or CD8+4-) thymocyte subsets compared to syngeneic total, mostly immature thymocytes. Whether these changes are induced by relatively weak superantigens or conventional antigens and whether the downshifts are caused by negative selection or lack of positive selection remains to be determined.

MeSH Terms
Child Child, Preschool Female Genetic Variation Humans Infant Infant, Newborn Macromolecular Substances Male RNA, Messenger/analysis,genetics Receptors, Antigen, T-Cell/genetics T-Lymphocyte Subsets/immunology T-Lymphocytes/immunology Thymus Gland/immunology Transcription, Genetic
Chemicals
Macromolecular Substances RNA, Messenger Receptors, Antigen, T-Cell
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Baccala R
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Kono D H
Walker S
Balderas R S
Theofilopoulos A N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.7.2908
Published
1991-04-01
Pages
2908-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51349
Subset
IM
Grants
NIAMS NIH HHS · AR31203 · United States
NIAMS NIH HHS · AR39555 · United States
NCI NIH HHS · CA27489 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.7.2908 PMC Full Text View journal details
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