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PMID: 2020540 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of the MboII restriction-modification system.

Nucleic acids research ·Vol. 19 ·No. 5 ·1991-03-11 ·Pages 1007-13

Bocklage H, Heeger K, Müller-Hill B

Abstract

The two genes encoding the class IIS restriction-modification system MboII from Moraxella bovis were cloned separately in two compatible plasmids and expressed in E. coli RR1 delta M15. The nucleotide sequences of the MboII endonuclease (R.MboII) and methylase (M.MboII) genes were determined and the putative start codon of R.MboII was confirmed by amino acid sequence analysis. The mboIIR gene specifies a protein of 416 amino acids (MW: 48,617) while the mboIIM gene codes for a putative 260-residue polypeptide (MW: 30,077). Both genes are aligned in the same orientation. The coding region of the methylase gene ends 11 bp upstream of the start codon of the restrictase gene. Comparing the amino acid sequence of M.MboII with sequences of other N6-adenine methyltransferases reveals a significant homology to M.RsrI, M.HinfI and M.DpnA. Furthermore, M.MboII shows homology to the N4-cytosine methyltransferase BamHI.

Related Genes
mboIIM mboIIR
MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Codon DNA, Bacterial Deoxyribonucleases, Type II Site-Specific/genetics,isolation & purification,metabolism Escherichia coli/genetics Exons Gene Expression Genes, Bacterial Molecular Sequence Data Moraxella/enzymology,genetics Plasmids Sequence Homology, Nucleic Acid Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics,isolation & purification,metabolism
Chemicals
Codon DNA, Bacterial DNA modification methylase MboII Site-Specific DNA-Methyltransferase (Adenine-Specific) endodeoxyribonuclease MboII Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bocklage H
Institut für Genetik, Universität zu Köln, FRG.
Heeger K
Müller-Hill B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.5.1007
Published
1991-03-11
Pages
1007-13
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333773
Subset
IM
Databases
GENBANK
S62405, S62419, S62424, S66489, X56307, X56310, X56977, X57453, X57454, X57455
External Links
PubMed source DOI: 10.1093/nar/19.5.1007 PMC Full Text View journal details
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