Home LiteratureArticle Details
PMID: 2118502 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical analysis and mapping of the Mycoplasma pneumoniae chromosome.

Journal of bacteriology ·Vol. 172 ·No. 9 ·1990-09-00 ·Pages 4790-7

Krause DC, Mawn CB

Abstract

Field inversion gel electrophoresis was used for analysis of the chromosome of Mycoplasma pneumoniae. The restriction endonuclease SfiI (5'-GGCCNNNNNGGCC-3') generated 2 M. pneumoniae DNA fragments of approximately 437 and 357.5 kilobase pairs (kbp), whereas 13 restriction fragments ranging in size from 2.4 to 252.0 kbp resulted from digestion with ApaI (5'-GGGCCC-3'). Totaling the sizes of the individual restriction fragments from digestion with SfiI or ApaI yielded a genome size of 794.5 or 775.4 kbp, respectively. A physical map of the M. pneumoniae chromosome was constructed by using a combination of techniques that included analysis by sequential or partial restriction endonuclease digestions and use as hybridization probes of cloned M. pneumoniae DNA containing ApaI sites and hence overlapping adjacent ApaI fragments. Genetic loci for deoC, rrn, hmw3, and the P1 gene were identified by using cloned DNA to probe ApaI restriction fragment profiles.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular DNA, Neoplasm/genetics,isolation & purification Genetic Linkage Mycoplasma pneumoniae/genetics Nucleic Acid Hybridization Plasmids Restriction Mapping
Chemicals
DNA, Neoplasm
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krause D C
Department of Microbiology, University of Georgia, Athens 30602.
Mawn C B
References (45)
45 references, click to expand
  1. Tissue cultures and mycoplasmas.
    Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ PMID: 5833547
  2. Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae.
    Gene. 1988 Dec 15;73(1):175-83 PMID: 2468577
  3. Analysis of the life cycle of Mycoplasma pneumoniae by synchronized division and by ultraviolet and x irradiations.
    J Infect Dis. 1968 Feb;118(1):7-13 PMID: 4867945
  4. Genome size of mycoplasmal DNA.
    Nature. 1969 Dec 20;224(5225):1209-10 PMID: 5358346
  5. Deoxyribonucleic acid synthesis in synchronously growing Mycoplasma gallisepticum.
    J Bacteriol. 1973 Jul;115(1):117-20 PMID: 4717510
  6. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium.
    J Exp Med. 1977 May 1;145(5):1328-43 PMID: 870608
  7. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  8. Size and physical map of the chromosome of Haemophilus influenzae.
    J Bacteriol. 1989 May;171(5):2474-9 PMID: 2785105
  9. Organization of the Haemophilus influenzae Rd genome.
    J Bacteriol. 1989 Jun;171(6):3016-24 PMID: 2785984
  10. Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.
    Nucleic Acids Res. 1989 May 11;17(9):3479-90 PMID: 2657661
  11. Genome organization of the anaerobic pathogen Clostridium perfringens.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6676-80 PMID: 2549543
  12. Cloning of the complete Mycoplasma pneumoniae genome.
    Nucleic Acids Res. 1989 Sep 12;17(17):7029-43 PMID: 2506532
  13. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence.
    Infect Immun. 1982 Mar;35(3):809-17 PMID: 6802761
  14. Molecular basis for cytadsorption of Mycoplasma pneumoniae.
    J Bacteriol. 1982 Sep;151(3):1514-22 PMID: 6809731
  15. Genomic and phenotypic analyses of Mycoplasma pneumoniae strains.
    Infect Immun. 1982 Nov;38(2):604-9 PMID: 6183217
  16. Reacquisition of specific proteins confers virulence in Mycoplasma pneumoniae.
    Infect Immun. 1983 Feb;39(2):830-6 PMID: 6403461
  17. Polyethylene glycol-dependent transfection of Acholeplasma laidlawii with mycoplasma virus L2 DNA.
    J Bacteriol. 1983 Aug;155(2):734-41 PMID: 6874642
  18. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
    Cell. 1984 May;37(1):67-75 PMID: 6373014
  19. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.
    Nucleic Acids Res. 1984 Jul 25;12(14):5647-64 PMID: 6379602
  20. Characterization of the mycoplasma genome.
    Yale J Biol Med. 1983 Sep-Dec;56(5-6):357-66 PMID: 6206652
  21. Cosmid cloning of Rickettsia prowazekii antigens in Escherichia coli K-12.
    Infect Immun. 1985 Jan;47(1):157-65 PMID: 2981194
  22. UGA is read as tryptophan in Mycoplasma capricolum.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2306-9 PMID: 3887399
  23. Molecular biology and genetics of mycoplasmas (Mollicutes).
    Microbiol Rev. 1985 Dec;49(4):419-55 PMID: 3912655
  24. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.
    Science. 1986 Apr 4;232(4746):65-8 PMID: 3952500
  25. Molecular analysis of the Duchenne muscular dystrophy region using pulsed field gel electrophoresis.
    Cell. 1987 Jan 30;48(2):351-7 PMID: 3026644
  26. Transposition of gram-positive transposon Tn916 in Acholeplasma laidlawii and Mycoplasma pulmonis.
    Science. 1987 Mar 13;235(4794):1392-4 PMID: 3029869
  27. A physical map of the Escherichia coli K12 genome.
    Science. 1987 Jun 12;236(4807):1448-53 PMID: 3296194
  28. Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis.
    J Bacteriol. 1987 Aug;169(8):3836-9 PMID: 3038852
  29. Preliminary indication of unusual codon usage in the DNA coding sequence of the attachment protein of Mycoplasma pneumoniae.
    Isr J Med Sci. 1987 May;23(5):361-7 PMID: 3117725
  30. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae.
    Infect Immun. 1987 Dec;55(12):3023-9 PMID: 3119495
  31. Pulsed-field electrophoresis: application of a computer model to the separation of large DNA molecules.
    Proc Natl Acad Sci U S A. 1987 Nov;84(22):8011-5 PMID: 3317398
  32. Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA.
    Nucleic Acids Res. 1988 Mar 25;16(5):2263-8 PMID: 2833729
  33. Pulsed-field gel electrophoresis of very large DNA molecules.
    Annu Rev Biophys Biophys Chem. 1988;17:287-304 PMID: 3293589
  34. Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.
    Nucleic Acids Res. 1988 Jul 11;16(13):6015-25 PMID: 2840639
  35. A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci.
    Nucleic Acids Res. 1988 Jul 11;16(13):6027-39 PMID: 2840640
  36. Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniae.
    Gene. 1988 Apr 29;64(2):217-29 PMID: 2841195
  37. Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis.
    J Bacteriol. 1988 Sep;170(9):4402-5 PMID: 2842319
  38. Physical mapping of the Mycoplasma pneumoniae genome.
    Nucleic Acids Res. 1988 Sep 12;16(17):8323-36 PMID: 2843814
  39. Repetitive DNA sequences in Mycoplasma pneumoniae.
    Nucleic Acids Res. 1988 Sep 12;16(17):8337-50 PMID: 3138660
  40. Regions of Mycoplasma pneumoniae cytadhesin P1 structural gene exist as multiple copies.
    Infect Immun. 1988 Dec;56(12):3157-61 PMID: 3141282
  41. Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.
    Nucleic Acids Res. 1988 Dec 23;16(24):11461-7 PMID: 3264905
  42. Nucleotide sequence of the deoC gene of Mycoplasma pneumoniae.
    Nucleic Acids Res. 1989 Jan 25;17(2):801 PMID: 2492658
  43. Anomalous values of Mycoplasma genomes sizes determined by pulse-field gel electrophoresis.
    Nucleic Acids Res. 1989 Feb 11;17(3):1268 PMID: 2922272
  44. Transformation of Mycoplasma pulmonis: demonstration of homologous recombination, introduction of cloned genes, and preliminary description of an integrating shuttle system.
    J Bacteriol. 1989 Apr;171(4):1775-80 PMID: 2539351
  45. Size and structure of the Mycoplasma hominis H39 chromosome.
    J Mol Biol. 1967 Jan 28;23(2):191-9 PMID: 6031715
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.9.4790-4797.1990
Published
1990-09-00
Pages
4790-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213132
Subset
IM
Grants
NIAID NIH HHS · R01 AI023362 · United States
NIAID NIH HHS · AI 23362 · United States
External Links
PubMed source DOI: 10.1128/jb.172.9.4790-4797.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2