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PMID: 2296087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptionally active genome regions are preferred targets for retrovirus integration.

Journal of virology ·Vol. 64 ·No. 2 ·1990-02-00 ·Pages 907-12

Scherdin U, Rhodes K, Breindl M

Abstract

We have analyzed the transcriptional activity of cellular target sequences for Moloney murine leukemia virus integration in mouse fibroblasts. At least five of the nine random, unselected integration target sequences studied showed direct evidence for transcriptional activity by hybridization to nuclear run-on transcripts prepared from uninfected cells. At least four of the sequences contained multiple recognition sites for several restriction enzymes that cut preferentially in CpG-rich islands, indicating integration into 5' or 3' ends or flanking regions of genes. Assuming that only a minor fraction (less than 20%) of the genome is transcribed in mammalian cells, we calculated the probability that this association of retroviral integration sites with transcribed sequences is due to chance to be very low (1.6 x 10(-2]. Thus, our results strongly suggest that transcriptionally active genome regions are preferred targets for retrovirus integration.

MeSH Terms
Animals Cells, Cultured Cloning, Molecular Dinucleoside Phosphates/analysis Genes, Viral Lysogeny Mice Moloney murine leukemia virus/genetics,physiology Plasmids Restriction Mapping Transcription, Genetic
Chemicals
Dinucleoside Phosphates cytidylyl-3'-5'-guanosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scherdin U
Department of Biology, San Diego State University, California 92182-0057.
Rhodes K
Breindl M
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47 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.2.907-912.1990
Published
1990-02-00
Pages
907-12
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249188
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.2.907-912.1990 PMC Full Text View journal details
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