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PMID: 2440176 Published · ppublish English Journal Article

Identification of the conserved, conformation-dependent cytokeratin epitope recognized by monoclonal antibody (lu-5).

Virchows Archiv. A, Pathological anatomy and histopathology ·Vol. 411 ·No. 2 ·1987-00-00 ·Pages 137-47

Franke WW, Winter S, von Overbeck J, Gudat F, Heitz PU, Stähli C

Abstract

The epitope recognized by the murine monoclonal antibody (mAB lu-5) recently described as a formaldehyde-resistant, "pan-epithelial marker" of great value in tumour diagnosis is located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution from amphibia to man. As this epitope is not reactive after SDS-polyacrylamide gel electrophoresis (SDS-PAGE), the epitope-bearing protein has been identified by a dot-blot antibody binding assay, using purified proteins in which the epitope is reconstituted. We show that the epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins. The location of this widespread epitope is discussed with respect to homologies of amino acid sequences of cytokeratins and their conformations.

MeSH Terms
Antibodies, Monoclonal/immunology Epitopes/immunology,isolation & purification Fluorescent Antibody Technique Humans Keratins/immunology Molecular Conformation
Chemicals
Antibodies, Monoclonal Epitopes Keratins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Franke W W
Winter S
von Overbeck J
Gudat F
Heitz P U
Stähli C
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Article Info
Journal
Virchows Archiv. A, Pathological anatomy and histopathology
Abbr.
Virchows Arch A Pathol Anat Histopathol
ISSN
0174-7398
DOI
10.1007/BF00712737
Published
1987-00-00
Pages
137-47
Language
English
Region
Germany
NLM ID
8302198
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00712737
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