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PMID: 25085909 Published · epublish English Journal Article Research Support, N.I.H., Extramural Review

End resection at double-strand breaks: mechanism and regulation.

Cold Spring Harbor perspectives in biology ·Vol. 6 ·No. 8 ·2014-08-01

Symington LS

Abstract

RecA/Rad51 catalyzed pairing of homologous DNA strands, initiated by polymerization of the recombinase on single-stranded DNA (ssDNA), is a universal feature of homologous recombination (HR). Generation of ssDNA from a double-strand break (DSB) requires nucleolytic degradation of the 5'-terminated strands to generate 3'-ssDNA tails, a process referred to as 5'-3' end resection. The RecBCD helicase-nuclease complex is the main end-processing machine in Gram-negative bacteria. Mre11-Rad50 and Mre11-Rad50-Xrs2/Nbs1 can play a direct role in end resection in archaea and eukaryota, respectively, by removing end-blocking lesions and act indirectly by recruiting the helicases and nucleases responsible for extensive resection. In eukaryotic cells, the initiation of end resection has emerged as a critical regulatory step to differentiate between homology-dependent and end-joining repair of DSBs.

MeSH Terms
Archaea/genetics Bacteria/genetics Chromatin Assembly and Disassembly DNA Breaks, Double-Stranded DNA Repair/physiology DNA, Single-Stranded/metabolism Eukaryota/genetics Models, Genetic
Chemicals
DNA, Single-Stranded
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Symington Lorraine S
Department of Microbiology and Immunology, Columbia University Medical Center, New York, New York 10032.
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Article Info
Journal
Cold Spring Harbor perspectives in biology
Abbr.
Cold Spring Harb Perspect Biol
ISSN
1943-0264
DOI
10.1101/cshperspect.a016436
Published
2014-08-01
Epub
2014-00-01
Language
English
Region
United States
NLM ID
101513680
PMCID
PMC4107989
Subset
IM
Grants
NCI NIH HHS · P01 CA174653 · United States
NIGMS NIH HHS · R01 GM041784 · United States
NIGMS NIH HHS · R01 GM094386 · United States
External Links
PubMed source DOI: 10.1101/cshperspect.a016436 PMC Full Text View journal details
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