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PMID: 2536832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of human papillomavirus type 18 transforming genes in immortalized and primary cells.

Journal of virology ·Vol. 63 ·No. 3 ·1989-03-00 ·Pages 1247-55

Bedell MA, Jones KH, Grossman SR, Laimins LA

Abstract

The selective retention and expression of the E6-E7 region of human papillomavirus (HPV) types 16 and 18 in cervical carcinomas suggests that these viral sequences play a role in the development of genital neoplasia. Each of three possible gene products, E6, E6*, and E7, from this region of HPV-18 were examined for transforming properties in several types of rodent cells. We have found that in immortalized fibroblasts, both E6 and E7 (but not E6*) are capable of inducing anchorage-independent growth. In rat embryo cells, the HPV-18 E7 open reading frame was an effective immortalizing agent and complemented an activated ras oncogene for transformation. In both immortalized and primary cells, transformation was observed when the HPV-18 sequences were expressed from either the HPV-18 promoter or a heterologous promoter. The E6-E7 region is not, however, the sole transforming domain of HPV-18, since another portion of the early region, possibly E5, also exhibited transforming capability in immortalized fibroblasts. The development of human cervical carcinomas may therefore involve a series of steps involving multiple viral and cellular gene products.

MeSH Terms
Animals Cell Line Cell Transformation, Viral Cloning, Molecular DNA Mutational Analysis DNA, Viral/genetics Genes, Viral Genes, ras Mice Mice, Nude Neoplasms, Experimental/microbiology Papillomaviridae/genetics Restriction Mapping
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bedell M A
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Jones K H
Grossman S R
Laimins L A
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.3.1247-1255.1989
Published
1989-03-00
Pages
1247-55
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247821
Subset
IM
Grants
NIGMS NIH HHS · 5T32 GM0782 · United States
NCI NIH HHS · CA 09273 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.3.1247-1255.1989 PMC Full Text View journal details
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