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PMID: 2753855 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Methylation of 23S rRNA caused by tlrA (ermSF), a tylosin resistance determinant from Streptomyces fradiae.

Journal of bacteriology ·Vol. 171 ·No. 8 ·1989-08-00 ·Pages 4254-60

Zalacain M, Cundliffe E

Abstract

Ribosomes from Streptomyces griseofuscus expressing tlrA, a resistance gene isolated from the tylosin producer Streptomyces fradiae, are resistant to macrolide and lincosamide antibiotics in vitro. The tlrA product was found to be a methylase that introduces two methyl groups into a single base within 23S rRNA, generating N6,N6-dimethyladenine at position 2058. This activity is therefore similar to the ermE resistance mechanism in Saccharopolyspora erythraea (formerly Streptomyces erythraeus).

MeSH Terms
Drug Resistance, Microbial/genetics Kinetics Leucomycins/pharmacology Methylation Methyltransferases/metabolism Plasmids Protein Biosynthesis RNA, Ribosomal/genetics RNA, Ribosomal, 23S/genetics,metabolism Ribosomes/metabolism Streptomyces/drug effects,genetics Transcription, Genetic Tylosin
Chemicals
Leucomycins RNA, Ribosomal RNA, Ribosomal, 23S Methyltransferases rRNA (adenosine-O-2'-)methyltransferase Tylosin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zalacain M
Department of Biochemistry, University of Leicester, United Kingdom.
Cundliffe E
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.8.4254-4260.1989
Published
1989-08-00
Pages
4254-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210198
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.8.4254-4260.1989 PMC Full Text View journal details
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