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PMID: 2850474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of a mouse VL30 retro-element.

Molecular and cellular biology ·Vol. 8 ·No. 8 ·1988-08-00 ·Pages 2989-98

Adams SE, Rathjen PD, Stanway CA, Fulton SM, Malim MH, Wilson W, Ogden J, King L, Kingsman SM, Kingsman AJ

Abstract

The complete nucleotide sequence of a mouse retro-element is presented. The cloned element is composed of 4,834 base pairs (bp) with long terminal repeats of 568 bp separated by an internal region of 3,698 bp. The element did not appear to have any open reading frames that would be capable of encoding the functional proteins that are normally produced by retro-elements. However, some regions of the genome showed some homology to retroviral gag and pol open reading frames. There was no region in VL30 corresponding to a retroviral env gene. This implies that VL30 is related to retrotransposons rather than to retroviruses. The sequence also contained regions that were homologous to known reverse transcriptase priming sites and viral packaging sites. These observations, combined with the known transcriptional capacity of the VL30 promoter, suggest that VL30 relies on protein functions of other retro-elements, such as murine leukemia virus, while maintaining highly conserved cis-active promoter, packaging, and priming sites necessary for its replication and cell-to-cell transmission.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Restriction Enzymes DNA, Viral/genetics Escherichia coli/genetics Mice Molecular Sequence Data Plasmids Retroviridae/genetics Sequence Homology, Nucleic Acid
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Adams S E
Department of Biochemistry, University of Oxford, United Kingdom.
Rathjen P D
Stanway C A
Fulton S M
Malim M H
Wilson W
Ogden J
King L
Kingsman S M
Kingsman A J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.8.2989-2998.1988
Published
1988-08-00
Pages
2989-98
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363524
Subset
IM
Databases
GENBANK
M21123
External Links
PubMed source DOI: 10.1128/mcb.8.8.2989-2998.1988 PMC Full Text View journal details
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