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PMID: 2864690 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The human Thy-1 gene: structure and chromosomal location.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 19 ·1985-10-00 ·Pages 6657-61

Seki T, Spurr N, Obata F, Goyert S, Goodfellow P, Silver J

Abstract

The human Thy-1 gene has been isolated and sequenced and compared to the rat and mouse Thy-1 genes. All three genes are organized in the same way: one exon encoding the majority of the signal peptide, another encoding the transmembrane segment, and a third encoding the remainder of the protein. One major structural difference between the human and rodent Thy-1 glycoproteins is that the former contains two instead of three glycosylation sites. RNA blot analysis of a human T-cell line expressing the T3 complex showed an absence of Thy-1 mRNA, excluding the possibility that Thy-1 represents one of the component chains of T3. The structural gene for human Thy-1 was localized to the long arm of chromosome 11 by nucleic acid hybridization to genomic DNA isolated from somatic cell hybrids.

MeSH Terms
Amino Acid Sequence Animals Antigens, Surface/genetics B-Lymphocytes/analysis Base Sequence Chromosome Mapping Chromosomes, Human, 6-12 and X DNA, Recombinant Genes Humans Mice/genetics Rats/genetics Recombinant Proteins/genetics Sequence Homology, Nucleic Acid Species Specificity Thy-1 Antigens
Chemicals
Antigens, Surface DNA, Recombinant Recombinant Proteins Thy-1 Antigens
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Seki T
Spurr N
Obata F
Goyert S
Goodfellow P
Silver J
References (43)
43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.19.6657
Published
1985-10-00
Pages
6657-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391269
Subset
IM
Databases
GENBANK
M11749
External Links
PubMed source DOI: 10.1073/pnas.82.19.6657 PMC Full Text View journal details
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