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PMID: 2884272 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Early genetic events in T cell development analyzed by in situ hybridization.

The Journal of experimental medicine ·Vol. 165 ·No. 6 ·1987-06-01 ·Pages 1624-38

Pardoll DM, Fowlkes BJ, Lechler RI, Germain RN, Schwartz RH

Abstract

In situ hybridization was used to investigate the expression of T cell receptor (TCR) alpha, beta, and gamma mRNAs in developing fetal and adult precursor thymocytes. gamma transcription was observed at the earliest time tested (day 12), followed by beta 12 h later, and TCR alpha on day 16. The early beta transcripts appeared to be from unrearranged or incompletely rearranged (D-J-C) beta loci. V beta region transcription was first detectable on day 14 and transcription of different V beta genes was induced at different times. These results delineate a schedule sequence of TCR gene activation, which begins within 1 d after entry of stem cells into the fetal thymus.

MeSH Terms
Animals Antigens, Surface/genetics Fetus/immunology,metabolism Mice Mice, Inbred C57BL Nucleic Acid Hybridization RNA, Messenger/analysis Receptors, Antigen, T-Cell/genetics Recombination, Genetic T-Lymphocytes/metabolism Thy-1 Antigens Thymus Gland/immunology Transcription, Genetic
Chemicals
Antigens, Surface RNA, Messenger Receptors, Antigen, T-Cell Thy-1 Antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pardoll D M
Fowlkes B J
Lechler R I
Germain R N
Schwartz R H
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.165.6.1624
Published
1987-06-01
Pages
1624-38
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188376
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.165.6.1624 PMC Full Text View journal details
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