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PMID: 2957691 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The RAD3 gene of Saccharomyces cerevisiae encodes a DNA-dependent ATPase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 17 ·1987-09-00 ·Pages 6045-9

Sung P, Prakash L, Weber S, Prakash S

Abstract

The RAD3 gene of the yeast Saccharomyces cerevisiae is required for excision repair of damaged DNA and for cell viability. A protein of approximately equal to 89 kDa was purified to near homogeneity from yeast strains harboring multicopy plasmids that overproduce RAD3 protein; this protein corresponds closely to the expected size of the RAD3 protein and cross-reacts with the antiserum raised against a truncated RAD3 protein produced in Escherichia coli. The purified RAD3 protein shows a single-stranded DNA-dependent ATPase activity that catalyzes hydrolysis of ATP to ADP and Pi. The ATPase activity was coincident with the RAD3 protein during purification and is inhibited by anti-RAD3 antibodies, indicating that the RAD3 gene encodes this activity.

MeSH Terms
Adenosine Triphosphatases/antagonists & inhibitors,genetics,isolation & purification Cloning, Molecular DNA Helicases DNA Repair Genes, Fungal Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Adenosine Triphosphatases DNA Helicases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sung P
Prakash L
Weber S
Prakash S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.17.6045
Published
1987-09-00
Pages
6045-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299004
Subset
IM
Grants
NCI NIH HHS · CA-35035 · United States
NIGMS NIH HHS · GM19261 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.17.6045 PMC Full Text View journal details
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