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PMID: 3097503 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A specific DNA sequence controls termination of transcription in the gastrin gene.

Molecular and cellular biology ·Vol. 6 ·No. 4 ·1986-04-00 ·Pages 1032-43

Sato K, Ito R, Baek KH, Agarwal K

Abstract

We located and characterized a downstream transcriptional regulatory element in the human gastrin gene by transferring the gastrin gene 3' fragment, from which the polyadenylation signal sequence was deleted, into the shuttle vector pSCAT10 at a site located immediately downstream from the chloramphenicol acetyltransferase (CAT) gene and upstream from the simian virus 40 polyadenylation region. Study of CAT RNA derived from the hybrid plasmids, indicated regulation of transcription on the gastrin gene fragment. Analysis of deletion mutants generated from the 5' region of the fragment by CAT assay and by S1 nuclease mapping of mRNAs indicated the possible involvement of an oligothymidylate-rich sequence in transcription regulation. Mapping of gastrin gene RNA 3' ends to the 5' side proximal to the oligothymidylate-rich sequence clearly demonstrated that this sequence is a transcriptional terminator element. This unique sequence, interspersed with one or two adenines, which also functions in an orientation-dependent manner, is located 192 nucleotides downstream from the gastrin gene polyadenylation site, and serves as a transcriptional termination signal.

MeSH Terms
Acetyltransferases/genetics Base Sequence Cell Line Chloramphenicol O-Acetyltransferase Chromosome Deletion Gastrins/genetics Genes Genes, Regulator Genetic Vectors Humans Mutation Plasmids Terminator Regions, Genetic Transcription, Genetic beta-Galactosidase/genetics
Chemicals
Gastrins Acetyltransferases Chloramphenicol O-Acetyltransferase beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sato K
Ito R
Baek K H
Agarwal K
References (46)
46 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.4.1032-1043.1986
Published
1986-04-00
Pages
1032-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367612
Subset
IM
Grants
NIADDK NIH HHS · AM21901 · United States
Databases
GENBANK
M12929
External Links
PubMed source DOI: 10.1128/mcb.6.4.1032-1043.1986 PMC Full Text View journal details
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