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PMID: 3866237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of sialic acid in kidney glomeruli: regionalization in the podocyte plasma membrane and loss in experimental nephrosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8508-12

Charest PM, Roth J

Abstract

Sialic acid residues were localized by electron microscopy in renal glomeruli of normal and puromycin-treated rats with a cytochemical technique that utilized the Limax flavus lectin. In Lowicryl K4M thin sections from normal rats, sialic acid residues were found along the plasma membrane of the various glomerular cell types and in the glomerular basement membrane as well as the mesangial matrix. In NaDodSO4/PAGE, sialic acid residues of normal glomeruli were mainly confined to a 140-kDa protein previously identified as podocalyxin. The distribution of sialic acid residues in the podocyte plasma membrane was found to be remarkably regionalized. Based on the differential labeling intensity, three plasma membrane domains could be defined: the foot process base, the foot process region above the slit diaphragm, and the body of podocytes. Cytochemical and biochemical analysis of glomeruli from puromycin-treated rats showed a loss of sialic acid residues from glomerular sialoglycoconjugates indicating a perturbated glycosylation.

MeSH Terms
Animals Cell Membrane/metabolism Gold Kidney Glomerulus/metabolism,ultrastructure Lectins Male Microscopy, Electron Molecular Weight Nephrosis/chemically induced,metabolism,pathology Plant Lectins Puromycin Rats Sialic Acids/metabolism Sialoglycoproteins/metabolism
Chemicals
Lectins Limax flavus agglutinin Plant Lectins Sialic Acids Sialoglycoproteins Puromycin Gold
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Charest P M
Roth J
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46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.24.8508
Published
1985-12-00
Pages
8508-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390945
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.24.8508 PMC Full Text View journal details
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