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PMID: 6091049 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Immunoglobulin genes of the kappa light chain type from two human lymphoid cell lines are closely related.

Nucleic acids research ·Vol. 12 ·No. 18 ·1984-09-25 ·Pages 6995-7006

Klobeck HG, Combriato G, Zachau HG

Abstract

As a first step in our studies of functionally rearranged K genes of man we cloned the germline JK-CK region from placenta DNA employing a mouse JK clone as hybridization probe. Subclones of the human JK-CK region were then used to characterize and clone the rearranged K genes of the lymphoid cell lines Walker and Daudi. The Walker cell line contains one rearranged and one germline K allele (K+,KO; ref. 1). Only one K gene was found in Daudi cells (K+). Restriction mapping and DNA sequencing showed, that the rearranged K genes from both cell lines are closely related. These features make the two cell lines particularly suitable for studies on the chromatin structure of K light chain genes. The 5' flanks of the two genes (388 bp) are identical while there is a 12% divergence between the VK gene segments themselves. This situation may reflect somatic mutation processes and/or gene conversion like events.

MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Burkitt Lymphoma/immunology Cell Line Cloning, Molecular DNA Restriction Enzymes DNA, Neoplasm/genetics Female Genes Humans Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Placenta/immunology Pregnancy
Chemicals
DNA, Neoplasm Immunoglobulin Light Chains Immunoglobulin kappa-Chains DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klobeck H G
Combriato G
Zachau H G
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.18.6995
Published
1984-09-25
Pages
6995-7006
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320138
Subset
IM
Databases
GENBANK
K02134, K02135
External Links
PubMed source DOI: 10.1093/nar/12.18.6995 PMC Full Text View journal details
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