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PMID: 6192448 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cDNA clone spanning the alpha-gamma subunit junction in the precursor of the murine fourth complement component (C4).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 16 ·1983-08-00 ·Pages 5061-5

Ogata RT, Shreffler DC, Sepich DS, Lilly SP

Abstract

cDNA clones carrying parts of murine fourth complement component (C4, serum substance protein) mRNA sequences have been identified by differential hybridization to mRNA from a high C4-producing strain, B10.WR, and a congeneic low C4 strain, B10.BR, followed by hybrid-selected translation and DNA sequence analysis. One clone, pMLC4/w7-2, encodes an open amino acid reading frame that includes four tandem arginine residues immediately preceding a sequence 85% homologous with the NH2-terminal sequence of the human C4 gamma-chain. The amino acid composition of the predicted sequence upstream of the tandem arginines matches quite closely with the composition of a similar sized peptide at the COOH terminus of the human C4 alpha chain. The latter result raises questions regarding the nature and extent of plasma-mediated postsynthetic processing of the C4 alpha-chain COOH terminus. The results also demonstrate that strain differences in plasma C4 levels (low C4 vs. high C4) reflect differences in steady-state levels of liver C4 mRNA in these strains.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Complement C4/genetics DNA/metabolism Humans Liver/immunology Macromolecular Substances Mice Mice, Inbred Strains Nucleic Acid Hybridization Poly A/genetics Protein Biosynthesis RNA/genetics RNA, Messenger/genetics Rabbits Reticulocytes/metabolism
Chemicals
Complement C4 Macromolecular Substances RNA, Messenger Poly A RNA DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ogata R T
Shreffler D C
Sepich D S
Lilly S P
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.16.5061
Published
1983-08-00
Pages
5061-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384188
Subset
IM
Grants
NIAID NIH HHS · AI12734 · United States
NIAID NIH HHS · AI15353 · United States
NIGMS NIH HHS · GM29831 · United States
Databases
GENBANK
K00019
External Links
PubMed source DOI: 10.1073/pnas.80.16.5061 PMC Full Text View journal details
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