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PMID: 6196189 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cytoplasmic RNA from normal and malignant human cells shows homology to the DNAs of Epstein-Barr virus and human adenoviruses.

The EMBO journal ·Vol. 2 ·No. 10 ·1983-00-00 ·Pages 1673-83

Arrand JR, Walsh-Arrand JE, Rymo L

Abstract

Cytoplasmic RNA prepared from several human cell lines and tissues was hybridised to DNA from Epstein-Barr virus, human adenovirus types 2, 3 and 12 and human papovaviruses BK and JC. RNA from all the cells, regardless of whether or not they were virally infected, hybridised to specific regions of the Epstein-Barr virus or adenovirus genomes but not to papovavirus DNA. The cellular cross-hybridising species appear to be repetitive sequences which are conserved in higher eukaryotes. Mismatch estimations indicate a high degree of homology between the viral and host sequences. Detailed analysis of selected regions of viral DNA failed to reveal any primary-structural peculiarities.

MeSH Terms
Adenoviruses, Human/genetics B-Lymphocytes/analysis Base Sequence Burkitt Lymphoma/analysis Cell Line DNA Restriction Enzymes DNA, Viral/genetics Herpesvirus 4, Human/genetics Humans Leukemia, Lymphoid/analysis Nucleic Acid Hybridization RNA/genetics RNA, Neoplasm/genetics Species Specificity Structure-Activity Relationship
Chemicals
DNA, Viral RNA, Neoplasm RNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arrand J R
Walsh-Arrand J E
Rymo L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1673-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555343
Subset
IM
Databases
GENBANK
V01509, V01554, V01555
External Links
PubMed source PMC Full Text View journal details
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