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PMID: 6273811 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene.

Nucleic acids research ·Vol. 9 ·No. 22 ·1981-11-25 ·Pages 5867-82

Sharp S, DeFranco D, Silberklang M, Hosbach HA, Schmidt T, Kubli E, Gergen JP, Wensink PC, Söll D

Abstract

We have isolated four segments of Drosophila melanogaster DNA that hybridize to homologous initiator tRNAMet. Three of the cloned fragments contain initiator tRNA genes, each of which can be transcribed in vitro. The fourth clone, pPW568, contains an initiator tRNA pseudogene which is not transcribed in vitro by RNA polymerase III. The pseudogene is contained in a 1.15 kb DNA fragment. This fragment has the characteristics of dispersed repetitive DNA and hybridizes in situ to at least 30 sites in the Drosophila genome. The arrangement of the initiator tRNA genes we have isolated, is different to that of other Drosophila tRNA gene families. The initiator tRNA genes are not clustered nor intermingled with other tRNA genes. They occur as single copies within an approximately 415-bp repeat segment, which is separated from other initiator tRNA genes by a mean distance of 17 kb. In situ hybridization to polytene chromosomes localizes these genes to the 61D region of the Drosophila genome. Hybridization analysis of genomic DNA indicates the presence of 8-9 non-allelic initiator tRNA genes in Drosophila melanogaster.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Drosophila melanogaster/genetics Genes Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Transfer/genetics Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Sharp S
DeFranco D
Silberklang M
Hosbach H A
Schmidt T
Kubli E
Gergen J P
Wensink P C
Söll D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.22.5867
Published
1981-11-25
Pages
5867-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327570
Subset
IM
Databases
GENBANK
K00460, K00461, K00462, V00247
External Links
PubMed source DOI: 10.1093/nar/9.22.5867 PMC Full Text View journal details
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