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PMID: 6328269 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription of the mouse ribosomal spacer region.

Molecular and cellular biology ·Vol. 4 ·No. 5 ·1984-05-00 ·Pages 822-8

Wood KM, Bowman LH, Thompson EA

Abstract

This paper describes experiments designed to test the hypothesis that DNA sequences upstream from the mouse rRNA promoter are transcribed in vivo or in vitro. Plasmid pB28 contains a SalI restriction fragment that extends from -169 to -1,894 base pairs, with respect to the origin of transcription of pre-rRNA. Labeled RNA synthesized in intact cells does not hybridize to this region. Neither S1 nuclease mapping nor RNA dot blot hybridization revealed the presence of sequences complementary to this region. Transcriptional studies carried out in vitro indicated that this region is not transcribed under conditions that are optimal for utilization of the authentic rRNA promoter. Moreover, this region does not appear to form stable transcription complexes with RNA polymerase I transcription components. These data indicate that the mouse rDNA repeating unit differs from those of Xenopus spp. and Drosophila melanogaster in that reduplicated RNA polymerase I promoters are not found in the mouse rDNA spacer region.

MeSH Terms

Animals Base Sequence Cell Line DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Endonucleases Lymphoma, Non-Hodgkin Mice Nucleic Acid Hybridization Operon Plasmids RNA, Ribosomal/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic

Chemicals

DNA, Ribosomal RNA, Ribosomal DNA Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wood K M

Bowman L H

Thompson E A

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24 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.4.5.822-828.1984

Published

1984-05-00

Pages

822-8

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC368820

Subset

Grants

NIADDK NIH HHS · AM32221 · United States

NCI NIH HHS · CA22394 · United States

NCI NIH HHS · CA24347 · United States

External Links

PubMed source DOI: 10.1128/mcb.4.5.822-828.1984 PMC Full Text View journal details

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Transcription of the mouse ribosomal spacer region.

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