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PMID: 6401281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutagenesis, by methylating and ethylating agents, in mutH, mutL, mutS, and uvrD mutants of Salmonella typhimurium LT2.

Journal of bacteriology ·Vol. 153 ·No. 1 ·1983-01-00 ·Pages 33-44

Shanabruch WG, Rein RP, Behlau I, Walker GC

Abstract

Salmonella typhimurium LT2 mutH, mutL, mutS, and uvrD mutants were especially sensitive to mutagenesis by both the recA+-dependent mutagen methyl methane sulfonate and the recA+-independent mutagen ethyl methane sulfonate, but not to mutagenesis by agents such as 4-nitroquinoline-1-oxide and UV irradiation. Similarly, these mutator strains were very sensitive to mutagenesis by the methylating agents N-methyl-N'-nitro-N-nitrosoguanidine and N-methyl-N-nitrosourea. The increased susceptibility to mutagenesis by small alkylating agents due to mutH, mutL, mutS, and uvrD mutations was not accompanied by an increased sensitivity to killing by these agents. Various models are discussed in an effort to explain why strains thought to be deficient in methyl-instructed mismatch repair are sensitive to mutagenesis by methylating and ethylating agents.

MeSH Terms
4-Nitroquinoline-1-oxide/pharmacology Alkylating Agents/pharmacology DNA Repair Ethyl Methanesulfonate/pharmacology Genes, Bacterial Methyl Methanesulfonate/pharmacology Methylnitronitrosoguanidine/pharmacology Methylnitrosourea/pharmacology Models, Genetic Mutagens/pharmacology Mutation Recombination, Genetic Salmonella typhimurium/drug effects,genetics Ultraviolet Rays
Chemicals
Alkylating Agents Mutagens Methylnitronitrosoguanidine 4-Nitroquinoline-1-oxide Methylnitrosourea Ethyl Methanesulfonate Methyl Methanesulfonate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shanabruch W G
Rein R P
Behlau I
Walker G C
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44 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.1.33-44.1983
Published
1983-01-00
Pages
33-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217339
Subset
IM
Grants
NCI NIH HHS · CA21615 · United States
NIGMS NIH HHS · GM07287 · United States
External Links
PubMed source DOI: 10.1128/jb.153.1.33-44.1983 PMC Full Text View journal details
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