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PMID: 7447923 Published · ppublish English Journal Article

Search for ribosomal mutants in Podospora anserina: genetic analysis of mutants resistant to paromomycin.

Biochemical genetics ·Vol. 18 ·No. 3-4 ·1980-04-00 ·Pages 263-80

Dequard M, Couderc JL, Legrain P, Belcour L, Picard-Bennoun M

Abstract

It has recently been shown that paromomycin, an antibiotic of the aminoglycoside family, is also active on eukaryotic cytoplasmic ribosomes. In the fungus Podospora anserina, genetic analysis of ten mutants resistant to high doses of paromomycin shows that this resistance is caused by mutations in two different nuclear genes. These mutants display pleiotropic phenotypes (cold sensitivity, mycelium and spore appearance and coloration, cross-resistance to other antibiotics). Double mutants are either lethal or very altered and unstable. Moreover, the cytochrome spectra of these mutants seem to indicate that cytoplasmic protein synthesis is affected. The mutants also display a slight suppressor effect. We can therefore assume that these mutations affect cytoplasmic ribosomes.

MeSH Terms
Ascomycota/drug effects,genetics,growth & development Cytochromes/biosynthesis Drug Resistance, Microbial Genes, Dominant Genetic Linkage Mutation Paromomycin/metabolism,pharmacology Protein Biosynthesis/drug effects Ribosomes Suppression, Genetic Temperature
Chemicals
Cytochromes Paromomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dequard M
Couderc J L
Legrain P
Belcour L
Picard-Bennoun M
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31 references, click to expand
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Article Info
Journal
Biochemical genetics
Abbr.
Biochem Genet
ISSN
0006-2928
DOI
10.1007/BF00484241
Published
1980-04-00
Pages
263-80
Language
English
Region
United States
NLM ID
0126611
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00484241 View journal details
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