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PMID: 7565750 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

YY1 facilitates the association of serum response factor with the c-fos serum response element.

Molecular and cellular biology ·Vol. 15 ·No. 11 ·1995-11-00 ·Pages 5975-82

Natesan S, Gilman M

Abstract

YY1 is a multifunctional transcription factor that acts as an activator or repressor in different contexts. YY1 binds to multiple sites in the mouse c-fos promoter, inducing at each site a sharp DNA bend. Binding of YY1 to a site situated between the cyclic AMP response element (CRE) and the TATA box bends the DNA in a way that interferes with the interaction of proteins bound at the CRE and TATA elements, resulting in repression of transcription. Here, we show that binding of YY1 to a different site in the c-fos promoter has a different result. Binding of YY1 to the c-fos serum response element (SRE) enhances the binding of serum response factor (SRF). This enhancement requires the binding of YY1 to SRE DNA. YY1 and SRF can cooccupy the SRE at least transiently. In the region of overlapping contact, YY1 contacts DNA in the major groove, while SRF contacts DNA in the minor groove. YY1 also enhances the association of SRF with the SRE in transfected insect cells. Thus, although YY1 induces similar structural changes in DNA at different binding sites, it can have distinct local effects on protein-DNA and protein-protein interactions. These data support a general role for YY1 in the building of highly organized promoter complexes.

MeSH Terms
Animals Base Sequence Binding Sites Cells, Cultured DNA-Binding Proteins/metabolism Drosophila melanogaster Erythroid-Specific DNA-Binding Factors Gene Expression Regulation Genes, fos Macromolecular Substances Mice Molecular Sequence Data Nuclear Proteins/metabolism Nucleic Acid Conformation Promoter Regions, Genetic Proto-Oncogene Proteins c-fos/genetics RNA, Messenger/genetics Serum Response Factor Transcription Factors/metabolism YY1 Transcription Factor
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Macromolecular Substances Nuclear Proteins Proto-Oncogene Proteins c-fos RNA, Messenger Serum Response Factor Transcription Factors YY1 Transcription Factor Yy1 protein, mouse
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Natesan S
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Gilman M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.11.5975
Published
1995-11-00
Pages
5975-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230849
Subset
IM
Grants
NCI NIH HHS · CA45642 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.11.5975 PMC Full Text View journal details
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