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PMID: 7622218 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of lbpA, the structural gene for a lactoferrin receptor in Neisseria gonorrhoeae.

Infection and immunity ·Vol. 63 ·No. 8 ·1995-08-00 ·Pages 2958-67

Biswas GD, Sparling PF

Abstract

Neisseria gonorrhoeae acquires iron (Fe) efficiently from lactoferrin (LF). A 103-kDa gonococcal outer membrane LF-binding protein (Lbp) was identified previously. We isolated the structural gene lbpA for Lbp1 by screening a gonococcal library for a clone that could repair an LF- receptor mutant. An mTnCm3 transposon insertion mutant of lbpA was unable to use LF-bound Fe for growth, unable to bind LF to whole cells, and unable to express Lbp1. The DNA sequence of lbpA predicted a protein that shared 94% identity with the meningococcal LF receptor protein, Lbp, and was closely related to Tbp1, one of the transferrin receptor proteins. Clinical isolates of gonococci are frequently unable to acquire Fe from LF, and LF- isolates do not have a functional LF receptor. The wild-type lbpA gene transformed most tested LF- clinical isolates to LF+, indicating that lbpA is defective in many clinical isolates.

Related Genes
lbpA
MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/chemistry,metabolism Base Sequence DNA Transposable Elements DNA, Bacterial/genetics Genes, Bacterial Lactoferrin/metabolism Molecular Sequence Data Mutagenesis Neisseria gonorrhoeae/genetics Receptors, Cell Surface/genetics Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Bacterial Outer Membrane Proteins DNA Transposable Elements DNA, Bacterial Receptors, Cell Surface lactoferrin receptors Lactoferrin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Biswas G D
Department of Medicine, University of North Carolina, Chapel Hill 27599, USA.
Sparling P F
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.63.8.2958-2967.1995
Published
1995-08-00
Pages
2958-67
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173402
Subset
IM
Grants
NCRR NIH HHS · 1S10RR05554-01 · United States
NIAID NIH HHS · AI26837 · United States
NIAID NIH HHS · AI31496 · United States
Databases
GENBANK
U16260
External Links
PubMed source DOI: 10.1128/iai.63.8.2958-2967.1995 PMC Full Text View journal details
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