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PMID: 7708500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cabeza, a Drosophila gene encoding a novel RNA binding protein, shares homology with EWS and TLS, two genes involved in human sarcoma formation.

Nucleic acids research ·Vol. 23 ·No. 5 ·1995-03-11 ·Pages 835-43

Stolow DT, Haynes SR

Abstract

We have previously described a partial Drosophila cDNA, clone P19, which bears homology to members of the RNA recognition motif (RRM) family of proteins [Haynes et al. (1987) Proc. Natl. Acad. Sci. USA, 84, 1819-1823]. RNA binding as well as involvement in RNA processing has been demonstrated for some RRM proteins. We report here the further characterization of P19, which we renamed cabeza (caz). caz is located on the X chromosome at position 14B. Using Northern analysis, at least four transcripts from the caz gene were observed at varying levels during development. caz mRNA and protein are enriched in the brain and central nervous system during embryogenesis. In addition, the protein is enriched in the adult head. UV crosslinking was used to demonstrate in vitro RNA binding activity for full-length recombinant caz protein and for the caz RRM domain. Sequence analysis revealed caz is related to two human genes, EWS and TLS, which are involved in chromosomal translocations. The fusion of EWS and TLS to other cellular genes results in sarcoma formation. In addition to their overall structural organization and sequence similarity, these three genes share an RRM which is divergent from typical RRMs. Therefore, it appears that these genes constitute a new sub-family of RNA binding proteins.

Related Genes
EWS TLS caz
MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA, Complementary Drosophila melanogaster/embryology,genetics Gene Expression Regulation, Developmental Heterogeneous-Nuclear Ribonucleoproteins Molecular Sequence Data Neoplasm Proteins/genetics Nuclear Proteins/genetics RNA, Messenger/genetics RNA-Binding Protein EWS RNA-Binding Protein FUS RNA-Binding Proteins/genetics Ribonucleoproteins/genetics Sarcoma/genetics
Chemicals
DNA, Complementary Heterogeneous-Nuclear Ribonucleoproteins Neoplasm Proteins Nuclear Proteins RNA, Messenger RNA-Binding Protein EWS RNA-Binding Protein FUS RNA-Binding Proteins Ribonucleoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stolow D T
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Haynes S R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.5.835
Published
1995-03-11
Pages
835-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306767
Subset
IM
Databases
GENBANK
L37083
External Links
PubMed source DOI: 10.1093/nar/23.5.835 PMC Full Text View journal details
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