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PMID: 7836295 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The export systems of type 1 and F1C fimbriae are interchangeable but work in parental pairs.

Journal of bacteriology ·Vol. 177 ·No. 3 ·1995-02-00 ·Pages 621-7

Klemm P, Jørgensen BJ, Kreft B, Christiansen G

Abstract

Type 1 and F1C fimbriae are surface organelles of Escherichia coli which mediate receptor-specific binding to different host surfaces. Such fimbriae are found, among others, on strains associated with urinary tract infections. Biosynthesis of type 1 and F1C fimbrial organelles requires individual, specialized two-component assembly systems. The organization of the fim and foc gene clusters encoding these fimbriae, as well as the structure of the organelles, is very similar; however, the actual sequence homology of the structural elements is not remarkable (34 to 60%). Both gene clusters encode a periplasmically located chaperone and an usher protein, located in the outer membrane, required for organelle biogenesis. Deletion of either element causes abolishment of fimbriation. The present report addresses the question of promiscuity in fimbrial biogenesis. Our data indicate that the two-component export systems of the two organelle systems are reciprocally interchangeable; however, they seem to function only in parental pairs.

MeSH Terms
Amino Acid Sequence Animals Bacterial Outer Membrane Proteins/genetics Bacterial Proteins Cell Line Escherichia coli Proteins Fimbriae Proteins Fimbriae, Bacterial/physiology Genes, Bacterial Guinea Pigs Humans Molecular Chaperones/physiology Molecular Sequence Data
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Proteins Molecular Chaperones fimC protein, E coli fimD protein, E coli fimD protein, bacteria Fimbriae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Klemm P
Department of Microbiology, Technical University of Denmark, Lyngby.
Jørgensen B J
Kreft B
Christiansen G
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.3.621-627.1995
Published
1995-02-00
Pages
621-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176636
Subset
IM
Databases
GENBANK
Z46635
External Links
PubMed source DOI: 10.1128/jb.177.3.621-627.1995 PMC Full Text View journal details
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