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PMID: 7935380 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter.

Molecular and cellular biology ·Vol. 14 ·No. 10 ·1994-10-00 ·Pages 6607-15

Neuman E, Flemington EK, Sellers WR, Kaelin WG

Abstract

The cell cycle-regulatory transcription factor E2F-1 is regulated by interactions with proteins such as the retinoblastoma gene product and by cell cycle-dependent alterations in E2F-1 mRNA abundance. To better understand this latter phenomenon, we have isolated the human E2F-1 promoter. The human E2F-1 promoter, fused to a luciferase cDNA, gave rise to cell cycle-dependent luciferase activity upon transfection into mammalian cells in a manner which paralleled previously reported changes in E2F-1 mRNA abundance. The E2F-1 promoter contains four potential E2F-binding sites organized as two imperfect palindromes. Gel shift and transactivation studies suggested that these sites can bind to E2F in vitro and in vivo. Mutation of the two E2F palindromes abolished the cell cycle dependence of the E2F-1 promoter. Thus, E2F-1 appears to be regulated at the level of transcription, and this regulation is due, at least in part, to binding of one or more E2F family members to the E2F-1 promoter.

MeSH Terms
Animals Base Sequence Carrier Proteins Cell Cycle/physiology Cell Cycle Proteins Cells, Cultured Cloning, Molecular DNA Mutational Analysis DNA-Binding Proteins/metabolism E2F Transcription Factors E2F1 Transcription Factor Humans Mice Molecular Sequence Data Polymerase Chain Reaction Promoter Regions, Genetic/genetics Protein Binding Recombinant Fusion Proteins/metabolism Retinoblastoma-Binding Protein 1 Sequence Analysis, DNA Sequence Deletion Single-Strand Specific DNA and RNA Endonucleases/metabolism Transcription Factor DP1 Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
Arid4a protein, mouse Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human E2f1 protein, mouse Recombinant Fusion Proteins Retinoblastoma-Binding Protein 1 Transcription Factor DP1 Transcription Factors Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Neuman E
Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Flemington E K
Sellers W R
Kaelin W G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.10.6607-6615.1994
Published
1994-10-00
Pages
6607-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359190
Subset
IM
Corrections
ErratumIn
PMID: 7623859
External Links
PubMed source DOI: 10.1128/mcb.14.10.6607-6615.1994 PMC Full Text View journal details
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