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PMID: 8083988 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Effects of human chromosome 12 on interactions between Tat and TAR of human immunodeficiency virus type 1.

Journal of virology ·Vol. 68 ·No. 10 ·1994-10-00 ·Pages 6505-13

Alonso A, Cujec TP, Peterlin BM

Abstract

Rates of transcriptions of the human immunodeficiency virus are greatly increased by the viral trans activator Tat. In vitro, Tat binds to the 5' bulge of the trans-activation response (TAR) RNA stem-loop, which is present in all viral transcripts. In human cells, the central loop in TAR and its cellular RNA-binding proteins are also critical for the function of Tat. Previously, we demonstrated that in rodent cells (CHO cells), but not in those which contain the human chromosome 12 (CHO12 cells), Tat-TAR interactions are compromised. In this study, we examined the roles of the bulge and loop in TAR in Tat trans activation in these cells. Whereas low levels of trans activation depended solely on interactions between Tat and the bulge in CHO cells, high levels of trans activation depended also on interactions between Tat and the loop in CHO12 cells. Since the TAR loop binding proteins in these two cell lines were identical and different from their human counterpart, the human chromosome 12 does not encode TAR loop binding proteins. In vivo binding competition studies with TAR decoys confirmed that the binding of Tat to TAR is more efficient in CHO12 cells. Thus, the protein(s) encoded on human chromosome 12 helps to tether Tat to TAR via its loop, which results in high levels of trans activation.

MeSH Terms
Animals Base Sequence Binding Sites CHO Cells Chloramphenicol O-Acetyltransferase/biosynthesis Chromosomes, Human, Pair 12 Cricetinae Gene Products, tat/metabolism HIV-1/genetics,metabolism HeLa Cells Humans Kinetics Molecular Sequence Data Nucleic Acid Conformation Plasmids RNA, Viral/biosynthesis,chemistry,metabolism Transcription, Genetic Transcriptional Activation Transfection tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat RNA, Viral tat Gene Products, Human Immunodeficiency Virus Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Alonso A
Department of Medicine, Howard Hughes Medical Institute, University of California at San Francisco 94143-0724.
Cujec T P
Peterlin B M
References (65)
65 references, click to expand
  1. A region of basic amino-acid cluster in HIV-1 Tat protein is essential for trans-acting activity and nucleolar localization.
    Virus Genes. 1989 Nov;3(2):99-110 PMID: 2559542
  2. Fragments of the HIV-1 Tat protein specifically bind TAR RNA.
    Science. 1990 Sep 14;249(4974):1281-5 PMID: 2205002
  3. Does the human immunodeficiency virus Tat trans-activator contain a discrete activation domain?
    Virology. 1990 Oct;178(2):560-7 PMID: 2219707
  4. Structural requirements for trans activation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by tat: importance of base pairing, loop sequence, and bulges in the tat-responsive sequence.
    J Virol. 1990 Mar;64(3):1402-6 PMID: 2406460
  5. Identification and characterization of a HeLa nuclear protein that specifically binds to the trans-activation-response (TAR) element of human immunodeficiency virus.
    Proc Natl Acad Sci U S A. 1990 May;87(9):3624-8 PMID: 2333305
  6. RNA transcripts of the human immunodeficiency virus transactivation response element can inhibit action of the viral transactivator.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5817-21 PMID: 1696012
  7. Human chromosome 12 encodes a species-specific factor which increases human immunodeficiency virus type 1 tat-mediated trans activation in rodent cells.
    J Virol. 1990 Sep;64(9):4565-7 PMID: 2200890
  8. Trans-activation by HIV-1 Tat via a heterologous RNA binding protein.
    Cell. 1990 Aug 24;62(4):769-76 PMID: 2117500
  9. Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replication.
    Cell. 1990 Nov 2;63(3):601-8 PMID: 2225067
  10. A bulge structure in HIV-1 TAR RNA is required for Tat binding and Tat-mediated trans-activation.
    Genes Dev. 1990 Aug;4(8):1365-73 PMID: 2227414
  11. HIV-1 tat protein stimulates transcription by binding to a U-rich bulge in the stem of the TAR RNA structure.
    EMBO J. 1990 Dec;9(12):4145-53 PMID: 2249668
  12. Synergy between HIV-1 Tat and adenovirus E1A is principally due to stabilization of transcriptional elongation.
    Genes Dev. 1990 Dec;4(12B):2397-408 PMID: 2149119
  13. Analysis of arginine-rich peptides from the HIV Tat protein reveals unusual features of RNA-protein recognition.
    Genes Dev. 1991 Feb;5(2):201-10 PMID: 1899841
  14. Cellular factors regulate transactivation of human immunodeficiency virus type 1.
    J Virol. 1991 Mar;65(3):1392-9 PMID: 1995949
  15. The acidic amino-terminal region of the HIV-1 Tat protein constitutes an essential activating domain.
    New Biol. 1989 Oct;1(1):101-10 PMID: 2562188
  16. Regulation of expression of human immunodeficiency virus.
    New Biol. 1990 Jan;2(1):20-31 PMID: 2078551
  17. Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.
    Science. 1991 Mar 29;251(5001):1597-600 PMID: 2011739
  18. The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):4045-9 PMID: 2023953
  19. Arginine-mediated RNA recognition: the arginine fork.
    Science. 1991 May 24;252(5009):1167-71 PMID: 1709522
  20. Tat-regulated production of multimerized TAR RNA inhibits HIV-1 gene expression.
    New Biol. 1991 Jan;3(1):82-9 PMID: 2039768
  21. Refolded HIV-1 tat protein protects both bulge and loop nucleotides in TAR RNA from ribonucleolytic cleavage.
    Biochemistry. 1991 Aug 13;30(32):8060-6 PMID: 1868081
  22. RNA recognition by Tat-derived peptides: interaction in the major groove?
    Cell. 1991 Aug 9;66(3):577-88 PMID: 1907891
  23. Critical chemical features in trans-acting-responsive RNA are required for interaction with human immunodeficiency virus type 1 Tat protein.
    J Virol. 1991 Oct;65(10):5196-202 PMID: 1895380
  24. tat regulates binding of the human immunodeficiency virus trans-activating region RNA loop-binding protein TRP-185.
    Genes Dev. 1991 Nov;5(11):2128-40 PMID: 1936997
  25. The nut site of bacteriophage lambda is made of RNA and is bound by transcription antitermination factors on the surface of RNA polymerase.
    Genes Dev. 1991 Nov;5(11):2141-51 PMID: 1834523
  26. Analysis of trans-acting response decoy RNA-mediated inhibition of human immunodeficiency virus type 1 transactivation.
    J Virol. 1991 Dec;65(12):6811-6 PMID: 1942253
  27. A minimal lentivirus Tat.
    J Virol. 1991 Dec;65(12):7012-5 PMID: 1658392
  28. Detailed mutational analysis of TAR RNA: critical spacing between the bulge and loop recognition domains.
    Nucleic Acids Res. 1991 Nov 25;19(22):6169-76 PMID: 1956776
  29. The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function.
    Genes Dev. 1991 Dec;5(12B):2496-507 PMID: 1752440
  30. Two distinct nuclear transcription factors recognize loop and bulge residues of the HIV-1 TAR RNA hairpin.
    Genes Dev. 1991 Dec;5(12B):2508-20 PMID: 1752441
  31. HIV-1 Tat protein promotes formation of more-processive elongation complexes.
    EMBO J. 1991 Dec;10(13):4189-96 PMID: 1756726
  32. Unusual structure of the human immunodeficiency virus type 1 trans-activation response element.
    J Virol. 1992 Feb;66(2):930-5 PMID: 1731114
  33. Binding of a host cell nuclear protein to the stem region of human immunodeficiency virus type 1 trans-activation-responsive RNA.
    J Virol. 1992 Mar;66(3):1688-94 PMID: 1738206
  34. HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors.
    Genes Dev. 1992 Apr;6(4):655-66 PMID: 1559613
  35. Conserved nucleotides in the TAR RNA stem of human immunodeficiency virus type 1 are critical for Tat binding and trans activation: model for TAR RNA tertiary structure.
    J Virol. 1992 May;66(5):3018-25 PMID: 1560535
  36. Human chromosome 12 is required for optimal interactions between Tat and TAR of human immunodeficiency virus type 1 in rodent cells.
    J Virol. 1992 Jul;66(7):4617-21 PMID: 1602563
  37. Conformation of the TAR RNA-arginine complex by NMR spectroscopy.
    Science. 1992 Jul 3;257(5066):76-80 PMID: 1621097
  38. Circular dichroism and molecular modeling yield a structure for the complex of human immunodeficiency virus type 1 trans-activation response RNA and the binding region of Tat, the trans-acting transcriptional activator.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9734-8 PMID: 1409690
  39. High affinity binding of TAR RNA by the human immunodeficiency virus type-1 tat protein requires base-pairs in the RNA stem and amino acid residues flanking the basic region.
    J Mol Biol. 1993 Mar 5;230(1):90-110 PMID: 8450553
  40. Role of RNA structure in arginine recognition of TAR RNA.
    Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3680-4 PMID: 7682716
  41. Juxtaposition between activation and basic domains of human immunodeficiency virus type 1 Tat is required for optimal interactions between Tat and TAR.
    J Virol. 1993 Jun;67(6):3441-5 PMID: 8497060
  42. TAR loop-dependent human immunodeficiency virus trans activation requires factors encoded on human chromosome 12.
    J Virol. 1993 Aug;67(8):5020-4 PMID: 8331737
  43. Modeling and solution structure probing of the HIV-1 TAR stem-loop.
    J Mol Graph. 1993 Jun;11(2):92-7, 124 PMID: 8347568
  44. Functional analysis of interactions between Tat and the trans-activation response element of human immunodeficiency virus type 1 in cells.
    J Virol. 1993 Sep;67(9):5617-22 PMID: 8350414
  45. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  46. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus.
    Nature. 1985 Feb 7-13;313(6002):450-8 PMID: 2982104
  47. The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.
    Cell. 1985 Jul;41(3):813-23 PMID: 2988790
  48. Expression and characterization of the trans-activator of HTLV-III/LAV virus.
    Science. 1986 Nov 21;234(4779):988-92 PMID: 3490693
  49. A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4831-5 PMID: 2440031
  50. Trans-activation of human immunodeficiency virus gene expression is mediated by nuclear events.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6364-8 PMID: 3476953
  51. Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product.
    Nature. 1987 Dec 3-9;330(6147):489-93 PMID: 2825027
  52. The cDNA for the beta-subunit of human chorionic gonadotropin suggests evolution of a gene by readthrough into the 3'-untranslated region.
    Nature. 1980 Aug 14;286(5774):684-7 PMID: 6774259
  53. Mutational analysis of the trans-activation-responsive region of the human immunodeficiency virus type I long terminal repeat.
    J Virol. 1988 Mar;62(3):673-9 PMID: 2828663
  54. Site-directed mutagenesis of two trans-regulatory genes (tat-III,trs) of HIV-1.
    Science. 1988 Feb 19;239(4842):910-3 PMID: 3277284
  55. HIV-1 tat trans-activation requires the loop sequence within tar.
    Nature. 1988 Jul 14;334(6178):165-7 PMID: 3386755
  56. Functional domains required for tat-induced transcriptional activation of the HIV-1 long terminal repeat.
    EMBO J. 1988 Oct;7(10):3143-7 PMID: 3181132
  57. Structural and functional characterization of human immunodeficiency virus tat protein.
    J Virol. 1989 Jan;63(1):1-8 PMID: 2535718
  58. Human immunodeficiency virus type 1 LTR TATA and TAR region sequences required for transcriptional regulation.
    EMBO J. 1989 Mar;8(3):765-78 PMID: 2721501
  59. Structure, sequence, and position of the stem-loop in tar determine transcriptional elongation by tat through the HIV-1 long terminal repeat.
    Genes Dev. 1989 Apr;3(4):547-58 PMID: 2470647
  60. Multiple functional domains of Tat, the trans-activator of HIV-1, defined by mutational analysis.
    Nucleic Acids Res. 1989 May 11;17(9):3551-61 PMID: 2542902
  61. Specific binding of a HeLa cell nuclear protein to RNA sequences in the human immunodeficiency virus transactivating region.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):4858-62 PMID: 2544877
  62. HIV-1 Tat protein increases transcriptional initiation and stabilizes elongation.
    Cell. 1989 Oct 20;59(2):283-92 PMID: 2553266
  63. Identification of cellular proteins that bind to the human immunodeficiency virus type 1 trans-activation-responsive TAR element RNA.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7828-32 PMID: 2510154
  64. Human chromosome 12 is required for elevated HIV-1 expression in human-hamster hybrid cells.
    Science. 1989 Oct 27;246(4929):488-91 PMID: 2683071
  65. trans activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: a quantitative analysis.
    J Virol. 1989 Dec;63(12):5501-4 PMID: 2479775
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.10.6505-6513.1994
Published
1994-10-00
Pages
6505-13
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237071
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.10.6505-6513.1994 PMC Full Text View journal details
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