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PMID: 8196609 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation.

Molecular and cellular biology ·Vol. 14 ·No. 6 ·1994-06-00 ·Pages 3634-45

Robinson MK, van Zyl WH, Phizicky EM, Broach JR

Abstract

The Saccharomyces cerevisiae TPD1 gene has been implicated in tRNA splicing because a tpd1-1 mutant strain accumulates unspliced precursor tRNAs at high temperatures (W. H. van Zyl, N. Wills, and J. R. Broach, Genetics 123:55-68, 1989). The wild-type TPD1 gene was cloned by complementation of the tpd1-1 mutation and shown to encode a protein with substantial homology to protein phosphatase 2C (PP2C) of higher eukaryotes. Expression of Tpd1p in Escherichia coli results in PP2C-like activity. Strains deleted for the TPD1 gene exhibit multiple phenotypes: temperature-sensitive growth, accumulation of unspliced precursor tRNAs, sporulation defects, and failure of cell separation during mitotic growth. On the basis of the presence of these observable phenotypes and the fact that Tpd1p accounts for a small percentage of the observed PP2C activity, we argue that Tpd1p is a unique member of the PP2C family.

Related Genes
LEU2-3 TPD1 lys14 tpd1 trp1
MeSH Terms
Amino Acid Sequence Cations/pharmacology Fungal Proteins/biosynthesis,genetics Genes, Fungal Genetic Complementation Test Genotype Kinetics Molecular Sequence Data Phosphoprotein Phosphatases/biosynthesis,genetics,metabolism Plasmids Protein Phosphatase 2 RNA Splicing RNA, Fungal/biosynthesis RNA, Transfer, Ser/biosynthesis Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
Cations Fungal Proteins RNA, Fungal RNA, Transfer, Ser Saccharomyces cerevisiae Proteins PTC1 protein, S cerevisiae Phosphoprotein Phosphatases Protein Phosphatase 2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Robinson M K
Department of Biochemistry, University of Rochester Medical School, New York 14642.
van Zyl W H
Phizicky E M
Broach J R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.6.3634-3645.1994
Published
1994-06-00
Pages
3634-45
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358731
Subset
IM
Grants
NIGMS NIH HHS · GM07102-17 · United States
NCI NIH HHS · NCI CA41086 · United States
External Links
PubMed source DOI: 10.1128/mcb.14.6.3634-3645.1994 PMC Full Text View journal details
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