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PMID: 1508188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing.

Molecular and cellular biology ·Vol. 12 ·No. 9 ·1992-09-00 ·Pages 3843-56

O'Connor JP, Peebles CL

Abstract

We have identified an essential Saccharomyces cerevisiae gene, PTA1, that affects pre-tRNA processing. PTA1 was initially defined by a UV-induced mutation, pta1-1, that causes the accumulation of all 10 end-trimmed, intron-containing pre-tRNAs and temperature-sensitive but osmotic-remedial growth. pta1-1 does not appear to be an allele of any other known gene affecting pre-tRNA processing. Extracts prepared from pta1-1 strains had normal pre-tRNA splicing endonuclease activity. pta1-1 was suppressed by the ochre suppressor tRNA gene SUP11, indicating that the pta1-1 mutation creates a termination codon within a protein reading frame. The PTA1 gene was isolated from a genomic library by complementation of the pta1-1 growth defect. Episome-borne PTA1 directs recombination to the pta1-1 locus. PTA1 has been mapped to the left arm of chromosome I near CDC24; the gene was sequenced and could encode a protein of 785 amino acids with a molecular weight of 88,417. No other protein sequences similar to that of the predicted PTA1 gene product have been identified within the EMBL or GenBank data base. Disruption of PTA1 near the carboxy terminus of the putative open reading frame was lethal. Possible functions of the PTA1 gene product are discussed.

MeSH Terms
Base Sequence DNA, Fungal Fungal Proteins/genetics Genes, Fungal Genes, Recessive Genetic Complementation Test Kinetics Molecular Sequence Data RNA Precursors/genetics,metabolism RNA Processing, Post-Transcriptional/genetics RNA Splicing RNA, Fungal/genetics,metabolism RNA, Transfer/genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins mRNA Cleavage and Polyadenylation Factors
Chemicals
DNA, Fungal Fungal Proteins PTA1 protein, S cerevisiae RNA Precursors RNA, Fungal Saccharomyces cerevisiae Proteins mRNA Cleavage and Polyadenylation Factors RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
O'Connor J P
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Peebles C L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.9.3843-3856.1992
Published
1992-09-00
Pages
3843-56
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360256
Subset
IM
Grants
NIGMS NIH HHS · GM37166 · United States
Databases
GENBANK
M95673
External Links
PubMed source DOI: 10.1128/mcb.12.9.3843-3856.1992 PMC Full Text View journal details
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