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PMID: 8223445 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Developmental and functional impairment of T cells in mice lacking CD3 zeta chains.

The EMBO journal ·Vol. 12 ·No. 11 ·1993-11-00 ·Pages 4357-66

Ohno H, Aoe T, Taki S, Kitamura D, Ishida Y, Rajewsky K, Saito T

Abstract

CD3 zeta is a component of the T cell antigen receptor (TCR) complex and is important for signal transduction. We have established mice selectively lacking CD3 zeta but able to express CD3 eta, a polypeptide produced from the same locus through alternative splicing, using the method of gene targeting in embryonic stem cells. In homozygous mutant mice, the numbers of thymocytes and peripheral T cells were greatly reduced and the expression levels of TCR on these cells were 5-fold lower than those on wild-type cells. By contrast, TCR gamma delta+ intestinal intraepithelial lymphocytes were not obviously affected by the mutation. T cells from homozygous mutants exhibited an impaired proliferative response. These results imply that CD3 zeta has a critical role in the development and signal transduction of T cells in vivo.

MeSH Terms
Animals Base Sequence Blood Cells/cytology,immunology CD3 Complex/genetics Homozygote Intestines/cytology,embryology,immunology Lymphocyte Activation Mice Mice, Inbred C57BL Molecular Sequence Data Receptors, Antigen, T-Cell/genetics Signal Transduction Stem Cells T-Lymphocytes/immunology Thymus Gland/cytology,embryology,immunology
Chemicals
CD3 Complex Receptors, Antigen, T-Cell
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ohno H
Division of Molecular Genetics, Chiba University, School of Medicine, Japan.
Aoe T
Taki S
Kitamura D
Ishida Y
Rajewsky K
Saito T
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47 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-11-00
Pages
4357-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413732
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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