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PMID: 8230482 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutants of Epstein-Barr virus with a selective marker disrupting the TP gene transform B cells and replicate normally in culture.

Journal of virology ·Vol. 67 ·No. 12 ·1993-12-00 ·Pages 7634-40

Kim OJ, Yates JL

Abstract

We have isolated mutants of Epstein-Barr virus (EBV) which carry a dominant selectable marker inserted into the third exon of the gene encoding two membrane proteins, TP1 and TP2 (or LMP2A and LMP2B), which are expressed in latently infected, growth-transformed B cells. One of the mutants also acquired a 260-bp deletion beginning in the first intron a few base pairs from the terminal repeats and removing most of the second TP exon, including the initial coding sequences of TP2. These EBV mutants transform human B cells in culture, and the transformed B-cell clones carrying them release EBV at approximately normal frequencies.

MeSH Terms
Amino Acid Sequence Antigens, Viral/genetics B-Lymphocytes/microbiology Base Sequence Cell Line, Transformed Cell Transformation, Viral/genetics Clone Cells Exons/genetics Genetic Markers Herpesvirus 4, Human/genetics,growth & development Humans Molecular Sequence Data Mutagenesis, Insertional Selection, Genetic Viral Matrix Proteins/genetics Virus Cultivation Virus Replication
Chemicals
Antigens, Viral EBV-associated membrane antigen, Epstein-Barr virus Genetic Markers Viral Matrix Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kim O J
Department of Human Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263-0001.
Yates J L
References (56)
56 references, click to expand
  1. Transforming functions associated with Epstein-Barr virus.
    J Invest Dermatol. 1984 Jul;83(1 Suppl):82s-87s PMID: 6330229
  2. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  3. Persistence of the entire Epstein-Barr virus genome integrated into human lymphocyte DNA.
    Science. 1984 Dec 14;226(4680):1322-5 PMID: 6095452
  4. Signals for site-specific cleavage of HSV DNA: maturation involves two separate cleavage events at sites distal to the recognition sequences.
    Cell. 1985 Jul;41(3):793-802 PMID: 2988788
  5. Activation of the major immediate early gene of human cytomegalovirus by cis-acting elements in the promoter-regulatory sequence and by virus-specific trans-acting components.
    J Virol. 1985 Aug;55(2):431-41 PMID: 2991567
  6. Activation of the pp60c-src kinase by middle T antigen binding or by dephosphorylation.
    EMBO J. 1985 Jun;4(6):1471-7 PMID: 2411538
  7. An EBV membrane protein expressed in immortalized lymphocytes transforms established rodent cells.
    Cell. 1985 Dec;43(3 Pt 2):831-40 PMID: 3000618
  8. Functional domains within the a sequence involved in the cleavage-packaging of herpes simplex virus DNA.
    J Virol. 1986 Sep;59(3):605-18 PMID: 3016323
  9. trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1.
    Mol Cell Biol. 1986 Nov;6(11):3838-46 PMID: 3025615
  10. Posttranslational processing of an Epstein-Barr virus-encoded membrane protein expressed in cells transformed by Epstein-Barr virus.
    J Virol. 1987 Mar;61(3):866-75 PMID: 3027413
  11. Posttranslational processing of the Epstein-Barr virus-encoded p63/LMP protein.
    J Virol. 1987 Jul;61(7):2100-8 PMID: 3035211
  12. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  13. Specificity of cleavage in replicative-form DNA of bovine herpesvirus 1.
    J Virol. 1988 Apr;62(4):1355-63 PMID: 2831397
  14. Transformation of Balb 3T3 cells by the BNLF-1 gene of Epstein-Barr virus.
    Oncogene. 1988 May;2(5):461-7 PMID: 2836780
  15. A spliced Epstein-Barr virus gene expressed in immortalized lymphocytes is created by circularization of the linear viral genome.
    EMBO J. 1988 Mar;7(3):769-74 PMID: 2840285
  16. Identification and characterization of oriLyt, a lytic origin of DNA replication of Epstein-Barr virus.
    Cell. 1988 Nov 4;55(3):427-33 PMID: 2846181
  17. A survey on intron and exon lengths.
    Nucleic Acids Res. 1988 Nov 11;16(21):9893-908 PMID: 3057449
  18. Two related Epstein-Barr virus membrane proteins are encoded by separate genes.
    J Virol. 1989 Feb;63(2):933-7 PMID: 2536113
  19. A promoter of Epstein-Barr virus that can function during latent infection can be transactivated by EBNA-1, a viral protein required for viral DNA replication during latent infection.
    J Virol. 1989 Jun;63(6):2644-9 PMID: 2542577
  20. Genetic analysis of immortalizing functions of Epstein-Barr virus in human B lymphocytes.
    Nature. 1989 Aug 3;340(6232):393-7 PMID: 2547164
  21. The terminal protein gene 2 of Epstein-Barr virus is transcribed from a bidirectional latent promoter region.
    J Gen Virol. 1989 Nov;70 ( Pt 11):3079-84 PMID: 2555438
  22. Epstein-Barr virus latent membrane protein (LMP1) and nuclear proteins 2 and 3C are effectors of phenotypic changes in B lymphocytes: EBNA-2 and LMP1 cooperatively induce CD23.
    J Virol. 1990 May;64(5):2309-18 PMID: 2157887
  23. Epstein-Barr virus latent membrane protein inhibits human epithelial cell differentiation.
    Nature. 1990 Apr 19;344(6268):777-80 PMID: 2158628
  24. Identification of Epstein-Barr virus terminal protein 1 (TP1) in extracts of four lymphoid cell lines, expression in insect cells, and detection of antibodies in human sera.
    J Virol. 1990 Jun;64(6):2759-67 PMID: 2159542
  25. Identification of the Epstein-Barr virus terminal protein gene products in latently infected lymphocytes.
    J Virol. 1990 Jun;64(6):2866-75 PMID: 2159547
  26. Morphological transformation of human keratinocytes expressing the LMP gene of Epstein-Barr virus.
    Nature. 1990 May 31;345(6274):447-9 PMID: 1692971
  27. Expression of the BNLF-1 oncogene of Epstein-Barr virus in the skin of transgenic mice induces hyperplasia and aberrant expression of keratin 6.
    Cell. 1990 Jun 29;61(7):1315-27 PMID: 1694724
  28. When Epstein-Barr virus persistently infects B-cell lines, it frequently integrates.
    J Virol. 1991 Mar;65(3):1245-54 PMID: 1847452
  29. Recombinant Epstein-Barr virus with small RNA (EBER) genes deleted transforms lymphocytes and replicates in vitro.
    Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1546-50 PMID: 1847527
  30. Epstein-Barr virus (EBV) recombinants: use of positive selection markers to rescue mutants in EBV-negative B-lymphoma cells.
    J Virol. 1991 Apr;65(4):1701-9 PMID: 1848303
  31. An Epstein-Barr virus protein associated with cell growth transformation interacts with a tyrosine kinase.
    J Virol. 1991 Jul;65(7):3681-92 PMID: 1710288
  32. The prototypical Epstein-Barr virus-transformed lymphoblastoid cell line IB4 is an unusual variant containing integrated but no episomal viral DNA.
    J Virol. 1991 Jul;65(7):3958-63 PMID: 1645805
  33. BHRF1, the Epstein-Barr virus gene with homology to Bc12, is dispensable for B-lymphocyte transformation and virus replication.
    J Virol. 1991 Nov;65(11):5991-6000 PMID: 1656084
  34. The Epstein-Barr virus nuclear protein encoded by the leader of the EBNA RNAs is important in B-lymphocyte transformation.
    J Virol. 1991 Dec;65(12):6826-37 PMID: 1658376
  35. BHRF1 of Epstein-Barr virus, which is homologous to human proto-oncogene bcl2, is not essential for transformation of B cells or for virus replication in vitro.
    J Virol. 1992 Apr;66(4):1899-906 PMID: 1312610
  36. Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts.
    J Virol. 1992 May;66(5):2689-97 PMID: 1313894
  37. Use of second-site homologous recombination to demonstrate that Epstein-Barr virus nuclear protein 3B is not important for lymphocyte infection or growth transformation in vitro.
    J Virol. 1992 May;66(5):2893-903 PMID: 1313908
  38. A selectable marker allows investigation of a nontransforming Epstein-Barr virus mutant.
    J Virol. 1992 May;66(5):3214-9 PMID: 1313925
  39. Consistent transcription of the Epstein-Barr virus LMP2 gene in nasopharyngeal carcinoma.
    J Virol. 1992 May;66(5):3257-62 PMID: 1313931
  40. Mammalian facilitative glucose transporter family: structure and molecular regulation.
    Annu Rev Physiol. 1992;54:911-30 PMID: 1562197
  41. Epstein-Barr virus latent gene expression in uncultured peripheral blood lymphocytes.
    J Virol. 1992 Jun;66(6):3715-24 PMID: 1316478
  42. Targeted gene disruption in Epstein-Barr virus.
    Virology. 1992 Jul;189(1):253-65 PMID: 1318603
  43. An Epstein-Barr virus transformation-associated membrane protein interacts with src family tyrosine kinases.
    J Virol. 1992 Aug;66(8):5161-7 PMID: 1321296
  44. Expression of the Epstein-Barr virus (EBV)-encoded membrane protein LMP1 impairs the in vitro growth, clonability and tumorigenicity of an EBV-negative Burkitt lymphoma line.
    Int J Cancer. 1992 Jul 30;51(6):949-55 PMID: 1322379
  45. The only domain which distinguishes Epstein-Barr virus latent membrane protein 2A (LMP2A) from LMP2B is dispensable for lymphocyte infection and growth transformation in vitro; LMP2A is therefore nonessential.
    J Virol. 1992 Nov;66(11):6461-9 PMID: 1328675
  46. The last seven transmembrane and carboxy-terminal cytoplasmic domains of Epstein-Barr virus latent membrane protein 2 (LMP2) are dispensable for lymphocyte infection and growth transformation in vitro.
    J Virol. 1993 Apr;67(4):2006-13 PMID: 8383224
  47. Molecular characterization of the dopamine transporter.
    Trends Pharmacol Sci. 1993 Feb;14(2):43-9 PMID: 8480373
  48. Epstein-Barr virus latent membrane protein 2A blocks calcium mobilization in B lymphocytes.
    J Virol. 1993 Jun;67(6):3087-94 PMID: 7684459
  49. Deletion of DNA encoding the first five transmembrane domains of Epstein-Barr virus latent membrane proteins 2A and 2B.
    J Virol. 1993 Aug;67(8):5068-74 PMID: 8392630
  50. Release of infectious Epstein-Barr virus by transformed marmoset leukocytes.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):190-4 PMID: 4346033
  51. Human lymphoblastoid cell lines derived from individuals without lymphoproliferative disease contain the same latent forms of Epstein-Barr virus DNA as those found in tumor cells.
    Int J Cancer. 1977 Aug 15;20(2):173-80 PMID: 197024
  52. Clonal transformation of adult human leukocytes by Epstein-Barr virus.
    J Virol. 1977 Sep;23(3):503-8 PMID: 197258
  53. The structure of the termini of the DNA of Epstein-Barr virus.
    Cell. 1979 Jul;17(3):661-71 PMID: 225039
  54. Epstein-Barr virus DNA is amplified in transformed lymphocytes.
    J Virol. 1979 Sep;31(3):590-5 PMID: 229241
  55. Cloning overlapping DNA fragments from the B95-8 strain of Epstein-Barr virus reveals a site of homology to the internal repetition.
    J Virol. 1981 Dec;40(3):977-82 PMID: 6275132
  56. DNA sequence analysis of the EcoRI Dhet fragment of B95-8 Epstein-Barr virus containing the terminal repeat sequences.
    Mol Biol Med. 1983 Nov;1(4):425-45 PMID: 6094955
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.12.7634-7640.1993
Published
1993-12-00
Pages
7634-40
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238232
Subset
IM
Grants
NCI NIH HHS · CA43122 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.12.7634-7640.1993 PMC Full Text View journal details
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