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PMID: 8413281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation.

Molecular and cellular biology ·Vol. 13 ·No. 11 ·1993-11-00 ·Pages 6876-88

Konopka JB

Abstract

Mating pheromone receptors activate a G-protein signaling pathway that induces changes in transcription, cell division, and morphogenesis needed for the conjunction of Saccharomyces cerevisiae. The C terminus of the alpha-factor pheromone receptor functions in two complex processes, adaptation and morphogenesis. Adaptation to alpha-factor may occur through receptor desensitization, and alpha-factor-induced morphogenesis forms the conjugation bridge between mating cells. A plasmid overexpression strategy was used to isolate a new gene, AFR1, which acts together with the receptor C terminus to promote adaptation. The expression of AFR1 was highly induced by alpha-factor. Unexpectedly, cells lacking AFR1 showed a defect in alpha-factor-stimulated morphogenesis that was similar to the morphogenesis defect observed in cells producing C-terminally truncated alpha-factor receptors. In contrast, AFR1 overexpression resulted in longer projections of morphogenesis, which suggests that this gene may directly stimulate morphogenesis. These results indicate that AFR1 encodes a developmentally regulated function that coordinates both the regulation of receptor signaling and the induction of morphogenesis during conjugation.

Related Genes
AFR1 MAT SST2 TRP1
MeSH Terms
Acclimatization Amino Acid Sequence Base Sequence Cell Division DNA, Fungal/analysis,genetics Fungal Proteins/biosynthesis,genetics,metabolism GTP-Binding Proteins/metabolism Gene Expression Regulation, Fungal/drug effects Genes, Fungal Genotype Kinetics Mating Factor Molecular Sequence Data Morphogenesis Peptides/pharmacology Pheromones/pharmacology Plasmids Receptors, Mating Factor Receptors, Peptide/metabolism Restriction Mapping Saccharomyces cerevisiae/cytology,genetics,physiology Saccharomyces cerevisiae Proteins Signal Transduction Transcription Factors Transcription, Genetic
Chemicals
AFR1 protein, S cerevisiae DNA, Fungal Fungal Proteins Peptides Pheromones Receptors, Mating Factor Receptors, Peptide Saccharomyces cerevisiae Proteins Transcription Factors Mating Factor GTP-Binding Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Konopka J B
Department of Microbiology, State University of New York at Stony Brook 11794-5222.
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.11.6876-6888.1993
Published
1993-11-00
Pages
6876-88
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364750
Subset
IM
Databases
GENBANK
L21702
External Links
PubMed source DOI: 10.1128/mcb.13.11.6876-6888.1993 PMC Full Text View journal details
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