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PMID: 8534909 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in nucleolar proteins lead to nucleolar accumulation of polyA+ RNA in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 6 ·No. 9 ·1995-09-00 ·Pages 1103-10

Kadowaki T, Schneiter R, Hitomi M, Tartakoff AM

Abstract

Synthesis of mRNA and rRNA occur in the chromatin-rich nucleoplasm and the nucleolus, respectively. Nevertheless, we here report that a Saccharomyces cerevisiae gene, MTR3, previously implicated in mRNA transport, codes for a novel essential 28-kDa nucleolar protein. Moreover, in mtr3-1 the accumulated polyA+ RNA actually colocalizes with nucleolar antigens, the nucleolus becomes somewhat disorganized, and rRNA synthesis and processing are inhibited. A strain with a ts conditional mutation in RNA polymerase I also shows nucleolar accumulation of polyA+ RNA, whereas strains with mutations in the nucleolar protein Nop1p do not. Thus, in several mutant backgrounds, when mRNA cannot be exported i concentrates in the nucleolus. mRNA may normally encounter nucleolar components before export and proteins such as Mtr3p may be critical for export of both mRNA and ribosomal subunits.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Cell Nucleolus/metabolism Cloning, Molecular Exonucleases Exosome Multienzyme Ribonuclease Complex Fungal Proteins/genetics,metabolism Genes, Fungal Molecular Sequence Data Nuclear Proteins/genetics,metabolism RNA Polymerase II/metabolism RNA, Fungal/metabolism RNA, Messenger/metabolism RNA, Ribosomal/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins Nuclear Proteins RNA, Fungal RNA, Messenger RNA, Ribosomal Saccharomyces cerevisiae Proteins RNA Polymerase II Exonucleases Exosome Multienzyme Ribonuclease Complex MTR3 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kadowaki T
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Schneiter R
Hitomi M
Tartakoff A M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.6.9.1103
Published
1995-09-00
Pages
1103-10
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301270
Subset
IM
Grants
NIDDK NIH HHS · DK-27651 · United States
NIGMS NIH HHS · GM-46569 · United States
Databases
GENBANK
S80548
External Links
PubMed source DOI: 10.1091/mbc.6.9.1103 PMC Full Text View journal details
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