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PMID: 12438427 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Gamma-herpesvirus latency is preferentially maintained in splenic germinal center and memory B cells.

The Journal of experimental medicine ·Vol. 196 ·No. 10 ·2002-11-18 ·Pages 1363-72

Flaño E, Kim IJ, Woodland DL, Blackman MA

Abstract

The gamma-herpesviruses are oncogenic B cell lymphotrophic viruses that establish life-long latency in the host. Murine gamma-herpesvirus 68 (MHV-68) infection of mice represents a unique system for analyzing gamma-herpesvirus latency in splenic B cells at different stages of infection. After intranasal infection with MHV-68 we analyzed the establishment of latency 14 days after infection, and the maintenance of latency 3 months after infection in different purified subpopulations of B cells in the spleen. The data show that MHV-68 latency is mainly established in germinal center B cells and that long-term latency is preferentially maintained in two different subsets of isotype-switched B cells, germinal center and memory B cells. Cell cycle analysis indicates that MHV-68 is located in both cycling and resting isotype-switched B cells. Analysis of viral gene expression showed that both lytic and latent viral transcripts were differentially expressed in germinal center and memory B cells during long-term latency. Together, these observations suggested that gamma-herpesviruses exploit the B cell life cycle in the spleen.

MeSH Terms
Animals B-Lymphocytes/immunology,virology Cell Cycle Flow Cytometry Gene Expression Profiling Herpesviridae/physiology Immunologic Memory Mice Polymerase Chain Reaction Spleen/virology Virus Latency
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Flaño Emilio
Trudeau Institute, Saranac Lake, NY 12983, USA.
Kim In-Jeong
Woodland David L
Blackman Marcia A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20020890
Published
2002-11-18
Pages
1363-72
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2193987
Subset
IM
Grants
NIAID NIH HHS · R01 AI042927 · United States
NIAID NIH HHS · AI42927 · United States
External Links
PubMed source DOI: 10.1084/jem.20020890 PMC Full Text View journal details
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