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PMID: 1316998 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expression of yeast cytochrome c1 is controlled at the transcriptional level by glucose, oxygen and haem.

Molecular & general genetics : MGG ·Vol. 232 ·No. 3 ·1992-04-00 ·Pages 447-59

Oechsner U, Hermann H, Zollner A, Haid A, Bandlow W

Abstract

The nuclear gene for cytochrome c1 in Saccharomyces cerevisiae (CYT1) was localized on chromosome XV. Its upstream region was identified by functional complementation. Fusion to the lacZ reporter gene on a CEN plasmid allowed study of the effect of carbon sources and of specific deletion mutations on expression of the gene in yeast transformants. Detailed promoter analysis combined with expression studies in recipient strains defective in regulatory genes identified cis-acting sites and transcription factors involved in the regulated expression of the cytochrome c1 gene. These analyses showed that, in the presence of glucose, transcription of CYT1 is positively controlled by oxygen, presumably through the haem signal, and mediated by the HAP1-encoded transactivator. It is additionally regulated by the HAP2/3/4 complex which mediates gene activation mainly under glucose-free conditions. Basal transcription is, in part, effected by CPF1, a centromere and promoter-binding factor.

Related Genes
CYT1 HAP1 HAP2 lacZ
MeSH Terms
Base Sequence Binding Sites Chromosome Deletion Chromosome Mapping Chromosomes, Fungal Cloning, Molecular Cytochromes c1/genetics,metabolism DNA, Fungal/genetics,isolation & purification Gene Expression Regulation, Fungal/drug effects Genes, Fungal Genes, Regulator Glucose/pharmacology Heme/pharmacology Molecular Sequence Data Oxygen/pharmacology Promoter Regions, Genetic Recombinant Fusion Proteins/metabolism Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/drug effects,genetics,metabolism Sequence Homology, Nucleic Acid Transcription Factors/metabolism Transcription, Genetic/drug effects Transcriptional Activation beta-Galactosidase/genetics,metabolism
Chemicals
DNA, Fungal Recombinant Fusion Proteins Transcription Factors Heme Cytochromes c1 beta-Galactosidase Glucose Oxygen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Oechsner U
Institut für Genetik und Mikrobiologie, München, FRG.
Hermann H
Zollner A
Haid A
Bandlow W
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00266250
Published
1992-04-00
Pages
447-59
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00266250
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