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PMID: 1374713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HOM/HOX homeobox genes are present in hydra (Chlorohydra viridissima) and are differentially expressed during regeneration.

The EMBO journal ·Vol. 11 ·No. 5 ·1992-05-00 ·Pages 1815-23

Schummer M, Scheurlen I, Schaller C, Galliot B

Abstract

Hydra, a diblastic animal consisting of two cell layers, ectoderm and endoderm, is one of the most ancient animals displaying an anteroposterior axis with a head and a foot developing from an uncommitted gastric region. As such, hydra is an interesting model for studying the presence and function of homeobox genes in a phylogenetically old organism. By screening a Chlorohydra viridissima cDNA library with a 'guessmer' oligonucleotide, we have cloned several such cnidarian homeobox-containing genes (cnox genes). Two of these, cnox1 and cnox2, display labial and Deformed type homeodomains respectively and could represent two ancestral genes of the HOM/HOX complexes; cnox3 exhibits some similarity to the BarH1 and the distal-less type homeodomains and a fourth gene is highly related to the msh/Hox7 type of homeodomain. We used quantitative PCR to study levels of expression of these genes along the body axis and during head regeneration. In all cases, the expression in heads was stronger than that in the gastric region. cnox1 transcripts dramatically peaked within the first hours of head regeneration, whereas cnox2 and cnox3 reached their maximal levels 1 and 2 days after cutting respectively. This differential expression of homeobox genes at various stages of regeneration suggests that they play specific roles in regenerative processes.

Related Genes
HOM HOX cnox msh
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA/genetics Gene Expression Genes, Homeobox Hydra/genetics,physiology Molecular Sequence Data Polymerase Chain Reaction RNA/genetics Regeneration Sequence Alignment Sequence Homology, Nucleic Acid
Chemicals
RNA DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schummer M
Zentrum für Molekulare Biologie, Heidelberg, FRG.
Scheurlen I
Schaller C
Galliot B
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55 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-05-00
Pages
1815-23
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556639
Subset
IM
Databases
GENBANK
X64625, X64626, X64627, X64628, X64629, X64700, X67723, X67724, X67725, X67726
External Links
PubMed source PMC Full Text View journal details
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