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PMID: 1709094 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

FGFR-4, a novel acidic fibroblast growth factor receptor with a distinct expression pattern.

The EMBO journal ·Vol. 10 ·No. 6 ·1991-06-00 ·Pages 1347-54

Partanen J, Mäkelä TP, Eerola E, Korhonen J, Hirvonen H, Claesson-Welsh L, Alitalo K

Abstract

We have previously identified two novel members of the fibroblast growth factor receptor (FGFR) gene family expressed in K562 erythroleukemia cells. Here we report cDNA cloning and analysis of one of these genes, named FGFR-4. The deduced amino acid sequence of FGFR-4 is 55% identical with both previously characterized FGFRs, flg and bek, and has the structural characteristics of a FGFR family member including three immunoglobulin-like domains in its extracellular part. Antibodies raised against the carboxy terminus of FGFR-4 detected 95 and 110 kd glycoproteins with a protein backbone of 88 kd in COS cells transfected with a FGFR-4 cDNA expression vector. The FGFR-4 protein expressed in COS cells could also be affinity-labeled with radioiodinated acidic FGF. Furthermore, ligand binding experiments demonstrated that FGFR-4 binds acidic FGF with high affinity but does not bind basic FGF. FGFR-4 is expressed as a 3.0 kb mRNA in the adrenal, lung, kidney, liver, pancreas, intestine, striated muscle and spleen tissues of human fetuses. The expression pattern of FGFR-4 is distinct from that of flg and bek and the yet additional member of the same gene family, FGFR-3, which we have also cloned from the K562 leukemia cells. Our results suggest that FGFR-4 along with other fibroblast growth factor receptors performs cell lineage and tissue-specific functions.

Related Genes
FGFR-4 FGRF-3 bek flg
MeSH Terms
Amino Acid Sequence Blotting, Northern Cloning, Molecular Cross-Linking Reagents DNA/genetics Fibroblast Growth Factor 1/metabolism Filaggrin Proteins Gene Expression Humans Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/genetics Receptors, Cell Surface/classification,genetics,metabolism Receptors, Fibroblast Growth Factor
Chemicals
Cross-Linking Reagents FLG protein, human Filaggrin Proteins RNA, Messenger Receptors, Cell Surface Receptors, Fibroblast Growth Factor Fibroblast Growth Factor 1 DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Partanen J
Department of Virology, University of Helsinki, Finland.
Mäkelä T P
Eerola E
Korhonen J
Hirvonen H
Claesson-Welsh L
Alitalo K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-06-00
Pages
1347-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452793
Subset
IM
Databases
GENBANK
X57205
External Links
PubMed source PMC Full Text View journal details
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