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PMID: 18539750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A cooperate in cell cycle-associated replication of peroxisomes.

The Plant cell ·Vol. 20 ·No. 6 ·2008-06-00 ·Pages 1567-85

Lingard MJ, Gidda SK, Bingham S, Rothstein SJ, Mullen RT, Trelease RN

Abstract

Although participation of PEROXIN11 (PEX11), FISSION1 (FISl), and DYNAMIN-RELATED PROTEIN (DRP) has been well established during induced peroxisome proliferation in response to external stimuli, their roles in cell cycle-associated constitutive replication/duplication have not been fully explored. Herein, bimolecular fluorescence complementation experiments with Arabidopsis thaliana suspension cells revealed homooligomerization of all five PEX11 isoforms (PEX11a-e) and heterooligomerizations of all five PEX11 isoforms with FIS1b, but not FIS1a nor DRP3A. Intracellular protein targeting experiments demonstrated that FIS1b, but not FIS1a nor DRP3A, targeted to peroxisomes only when coexpressed with PEX11d or PEX11e. Simultaneous silencing of PEX11c-e or individual silencing of DRP3A, but not FIS1a nor FIS1b, resulted in approximately 40% reductions in peroxisome number. During G2 in synchronized cell cultures, peroxisomes sequentially enlarged, elongated, and then doubled in number, which correlated with peaks in PEX11, FIS1, and DRP3A expression. Overall, these data support a model for the replication of preexisting peroxisomes wherein PEX11c, PEX11d, and PEX11e act cooperatively during G2 to promote peroxisome elongation and recruitment of FIS1b to the peroxisome membrane, where DRP3A stimulates fission of elongated peroxisomes into daughter peroxisomes, which are then distributed between daughter cells.

MeSH Terms
Arabidopsis/cytology,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Cell Cycle/genetics,physiology Cells, Cultured Membrane Proteins/genetics,metabolism Microscopy, Fluorescence Peroxins Peroxisomes/metabolism Protein Isoforms/genetics,metabolism RNA Interference Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Arabidopsis Proteins Membrane Proteins Peroxins Protein Isoforms dynamin-related protein DRP3A, Arabidopsis peroxin IIb, Arabidopsis
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lingard Matthew J
School of Life Sciences, Arizona State University, Tempe, Arizona 85287-4501, USA.
Gidda Satinder K
Bingham Scott
Rothstein Steven J
Mullen Robert T
Trelease Richard N
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.057679
Published
2008-06-00
Epub
2008-00-06
Pages
1567-85
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2483373
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.107.057679 PMC Full Text View journal details
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