Home LiteratureArticle Details
PMID: 1860815 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Saturation mutagenesis of the UASNTR (GATAA) responsible for nitrogen catabolite repression-sensitive transcriptional activation of the allantoin pathway genes in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 173 ·No. 16 ·1991-08-00 ·Pages 4977-82

Bysani N, Daugherty JR, Cooper TG

Abstract

Saturation mutagenesis of the UASNTR element responsible for GLN3-dependent, nitrogen catabolite repression-sensitive transcriptional activation of allantoin pathway genes in yeast cells identified the dodecanucleotide sequence 5'-TTNCTGATAAGG-3' as the minimum required for UAS activity. There was significant flexibility in mutant sequences capable of supporting UAS activity, which correlates well with the high variation in UASNTR homologous sequences reported to be upstream of the DAL and DUR genes. Three of nine UASNTR-like sequences 5' of the DAL5 gene supported high-level transcriptional activation. The others, which contained nonpermissive substitutions, were not active.

MeSH Terms
Allantoin/genetics,metabolism Base Sequence Gene Expression Regulation, Fungal Genes, Fungal/genetics Genes, Regulator/genetics Molecular Sequence Data Mutagenesis Nitrogen/metabolism Promoter Regions, Genetic/genetics Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics,metabolism Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Allantoin Nitrogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bysani N
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Daugherty J R
Cooper T G
References (39)
39 references, click to expand
  1. Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
    Methods Enzymol. 1983;101:181-91 PMID: 6310321
  2. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  3. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  4. Oxaluric acid: a non-metabolizable inducer of the allantoin degradative enzymes in Saccharomyces cerevisiae.
    J Bacteriol. 1974 Mar;117(3):1240-7 PMID: 4591950
  5. Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2340-4 PMID: 4599622
  6. Isolation of the CAR1 gene from Saccharomyces cerevisiae and analysis of its expression.
    Mol Cell Biol. 1982 Dec;2(12):1514-23 PMID: 14582193
  7. DAL82, a second gene required for induction of allantoin system gene transcription in Saccharomyces cerevisiae.
    J Bacteriol. 1991 Jan;173(1):255-61 PMID: 1898922
  8. Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID.
    Genes Dev. 1990 Jul;4(7):1141-8 PMID: 2210373
  9. Nuclear proteins that bind the human gamma-globin gene promoter: alterations in binding produced by point mutations associated with hereditary persistence of fetal hemoglobin.
    Mol Cell Biol. 1988 Dec;8(12):5310-22 PMID: 2468996
  10. An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5976-80 PMID: 3413070
  11. Expression of the SV40 promoter in fission yeast: identification and characterization of an AP-1-like factor.
    Cell. 1988 May 20;53(4):659-67 PMID: 2836064
  12. Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae.
    Eur J Biochem. 1985 Jun 3;149(2):401-4 PMID: 3888627
  13. Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.
    Mol Cell Biol. 1987 Mar;7(3):1256-66 PMID: 2951591
  14. Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4.
    Cell. 1988 Apr 22;53(2):321-30 PMID: 2834068
  15. Co-ordinate control of synthesis of mitochondrial and non-mitochondrial hemoproteins: a binding site for the HAP1 (CYP1) protein in the UAS region of the yeast catalase T gene (CTT1).
    EMBO J. 1988 Jun;7(6):1799-804 PMID: 2844525
  16. Induction and repression of the urea amidolyase gene in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Nov;6(11):3954-64 PMID: 3025621
  17. Nucleotide sequence of the GDH gene coding for the NADP-specific glutamate dehydrogenase of Saccharomyces cerevisiae.
    Gene. 1985;37(1-3):247-53 PMID: 2932370
  18. Proline utilization in Saccharomyces cerevisiae: sequence, regulation, and mitochondrial localization of the PUT1 gene product.
    Mol Cell Biol. 1987 Dec;7(12):4431-40 PMID: 3125423
  19. Novel Jun- and Fos-related proteins in Drosophila are functionally homologous to enhancer factor AP-1.
    EMBO J. 1988 Dec 20;7(13):4265-73 PMID: 3149584
  20. A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box.
    Mol Cell Biol. 1988 Nov;8(11):4634-41 PMID: 3062363
  21. The human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein.
    Genes Dev. 1988 Sep;2(9):1089-100 PMID: 2461328
  22. Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription.
    Mol Gen Genet. 1989 Jun;217(2-3):370-7 PMID: 2570348
  23. The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression.
    Mol Cell Biol. 1989 Aug;9(8):3231-43 PMID: 2552287
  24. Requirement of upstream activation sequences for nitrogen catabolite repression of the allantoin system genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Dec;9(12):5440-4 PMID: 2511434
  25. A gene product needed for induction of allantoin system genes in Saccharomyces cerevisiae but not for their transcriptional activation.
    Mol Cell Biol. 1989 Sep;9(9):3869-77 PMID: 2674683
  26. Identification of sequences responsible for transcriptional activation of the allantoate permease gene in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Feb;9(2):602-8 PMID: 2651902
  27. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  28. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements.
    Cell. 1987 Dec 4;51(5):721-32 PMID: 3315231
  29. Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae.
    J Bacteriol. 1987 Aug;169(8):3521-4 PMID: 3301804
  30. Ubiquitous upstream repression sequences control activation of the inducible arginase gene in yeast.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):3997-4001 PMID: 3295874
  31. Molecular characterization of the CAN1 locus in Saccharomyces cerevisiae. A transmembrane protein without N-terminal hydrophobic signal sequence.
    J Biol Chem. 1985 Sep 25;260(21):11831-7 PMID: 3900064
  32. The GLN3 gene product is required for transcriptional activation of allantoin system gene expression in Saccharomyces cerevisiae.
    J Bacteriol. 1990 Feb;172(2):1014-8 PMID: 2153652
  33. Functional domains and upstream activation properties of cloned human TATA binding protein.
    Science. 1990 Jun 29;248(4963):1625-30 PMID: 2363050
  34. A cis-acting element present in multiple genes serves as a repressor protein binding site for the yeast CAR1 gene.
    Mol Cell Biol. 1990 Aug;10(8):3884-95 PMID: 2115115
  35. nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein.
    Proc Natl Acad Sci U S A. 1990 Jul;87(14):5331-5 PMID: 2142530
  36. GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression.
    Eur J Biochem. 1990 May 31;190(1):39-44 PMID: 2194797
  37. Cloning of a transcriptionally active human TATA binding factor.
    Science. 1990 Jun 29;248(4963):1646-50 PMID: 2194289
  38. Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells.
    Biochem Biophys Res Commun. 1990 Jan 15;166(1):324-9 PMID: 2405848
  39. A nonerythroid GATA-binding protein is required for function of the human preproendothelin-1 promoter in endothelial cells.
    Mol Cell Biol. 1990 Sep;10(9):4854-62 PMID: 2388628
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.16.4977-4982.1991
Published
1991-08-00
Pages
4977-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208186
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM-35642 · United States
External Links
PubMed source DOI: 10.1128/jb.173.16.4977-4982.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2