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PMID: 1963869 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transfer RNA genes are genomic targets for de Novo transposition of the yeast retrotransposon Ty3.

Genetics ·Vol. 126 ·No. 4 ·1990-12-00 ·Pages 837-50

Chalker DL, Sandmeyer SB

Abstract

Insertions of the yeast element Ty3 resulting from induced retrotransposition were characterized in order to identify the genomic targets of transposition. The DNA sequences of the junctions between Ty3 and flanking DNA were determined for two insertions of an unmarked element. Each insertion was at position -17 from the 5' end of a tRNA-coding sequence. Ninety-one independent insertions of a marked Ty3 element were studied by Southern blot analysis. Pairs of independent insertions into seven genomic loci accounted for 14 of these insertions. The DNA sequence flanking the insertion site was determined for at least one member of each pair of integrated elements. In each case, insertion was at position -16 or -17 relative to the 5' end of one of seven different tRNA genes. This proportion of genomic loci used twice for Ty3 integration is consistent with that predicted by a Poisson distribution for a number of genomic targets roughly equivalent to the estimated number of yeast tRNA genes. In addition, insertions upstream of the same tRNA gene in one case were at different positions, but in all cases were in the same orientation. Thus, genomic insertions of Ty3 in a particular orientation are apparently specified by the target, while the actual position of the insertion relative to the tRNA-coding sequence can vary slightly.

Related Genes
URA3
MeSH Terms
Base Sequence Blotting, Southern Cloning, Molecular DNA Transposable Elements Galactose/metabolism Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Mutation RNA, Fungal/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/genetics Transformation, Genetic
Chemicals
DNA Transposable Elements RNA, Fungal RNA, Transfer Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chalker D L
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
Sandmeyer S B
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/126.4.837
Published
1990-12-00
Pages
837-50
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1204282
Subset
IM
Grants
NIGMS NIH HHS · GM07134 · United States
NIGMS NIH HHS · GM33281 · United States
External Links
PubMed source DOI: 10.1093/genetics/126.4.837 PMC Full Text View journal details
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